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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Milk fat protects Bifidobacterium animalis subsp lactis Bb-12 from in vitro gastrointestinal stress in potentially synbiotic table spreads

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Author(s):
dos Santos, Clara Simone [1] ; Batista de Souza, Cinthia Hoch [1, 2] ; Padilha, Marina [3, 1] ; Gioielli, Luiz Antonio [1] ; Rodrigues Ract, Juliana Neves [1] ; Saad, Susana Marta Isay [3, 1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Av Prof Lineu Prestes 580, B16, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ North Parana, Dairy Sci & Technol, Rua Marselha 591, BR-86041140 Londrina, PR - Brazil
[3] Univ Sao Paulo, Food Res Ctr, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FOOD & FUNCTION; v. 9, n. 8, p. 4274-4281, AUG 1 2018.
Web of Science Citations: 2
Abstract

The viability and the in vitro gastrointestinal survival of Bifidobacterium animalis subsp. lactis Bb-12 (Bifidobacterium Bb-12) in table spreads with different proportions of milk fat (MF) and palm olein (PO) (MF : PO 40 : 60 and MF : PO 20 : 80) were investigated for up to 28 days of storage at 5 degrees C. Moreover, qPCR alone and combined with propidium monoazide (PMA) were compared with the traditional plate count method for determining the in vitro gastrointestinal survival of Bifidobacterium Bb-12 in table spreads after 35 days of storage. Formulations showed probiotic viabilities ranging from 8 to 9 log CFU g(-1) across the whole storage period, and the milk fat and palm olein in different concentrations did not affect this viability. Bifidobacterium Bb-12 showed good survival after six hours under in vitro simulated gastrointestinal conditions during the studied storage period, with average reductions of 1.70 (MF : PO 40 : 60) and 2.16 log CFU g(-1) (MF : PO 20 : 80). The results of the qPCR with PMA treatment and the plate count method were similar and the qPCR without PMA treatment was shown to overestimate the Bifidobacterium Bb-12 populations. However, the MF : PO 40 : 60 spread showed a Bb-12 population between 0.76 and 1.43 log CFU g(-1) higher than that of MF : PO 20 : 80. Thus, the results showed that table spreads, especially food matrices with a higher proportion of milk fat, are suitable for the incorporation of Bifidobacterium Bb-12. (AU)

FAPESP's process: 14/04784-9 - Improvement of probiotic margarine sensory features and evaluation of the product functional potential
Grantee:Clara Simone dos Santos
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/07914-8 - FoRC - Food Research Center
Grantee:Bernadette Dora Gombossy de Melo Franco
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 15/15863-0 - Application of structured lipids for obtaining a spread as a probiotic and prebiotic functional food
Grantee:Juliana Neves Rodrigues Ract
Support type: Regular Research Grants