Use of zein microspheres to anchor carbon black and hemoglobin in electrochemical biosensors to detect hydrogen peroxide in cosmetic products, food and biological fluids

Author(s)

Pereira, Tamires dos Santos ^[1] ; Mauruto de Oliveira, Gabriela C. ^[1] ; Santos, Fabricio Aparecido ^[2] ; Raymundo-Pereira, Paulo A. ^[2] ; Oliveira, Jr., Osvaldo N. ^[2] ; Janegitz, Bruno Campos ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13563120 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Talanta; v. 194, p. 737-744, MAR 1 2019.
• Web of Science Citations: 5

Abstract

Hemoglobin-containing electrochemical biosensors are useful for detecting hydrogen peroxide through oxidation of the iron ion, but high efficiency can only be reached with appropriate immobilization strategies for hemoglobin. In this work, we combined zein from corn seed with carbon black to immobilize hemoglobin, as proof of concept, and form an electroactive film that could determine hydrogen peroxide within the concentration range from 4.9 x 10(-6) to 3.9 x 10(-4) mo L-1, and limit of detection of 4.0 x 10(-6) mol L-1, using differential pulse voltammetry. The biosensor could also detect hydrogen peroxide in commercial samples of oxygenated water, synthetic serum (physiological and glycoside) and milk. The high performance is ascribed to the large surface area and conductive nature of the porous film that had carbon black and hemoglobin anchored on zein microspheres, according to scanning and transmission electron microscopies. It is significant that a protein from renewable sources (zein) combined with a low-cost carbon material (carbon black) serves as matrix for immobilization of biomolecules. (AU)

• FAPESP's process: 17/17572-8 - Development of imunossensor and electrochemical sensor for detection of clinical character analyzes
• Grantee: Gabriela Carolina Mauruto de Oliveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/01919-6 - Design and fabrication of nanostructured flexible devices for biomarkers detection
• Grantee: Paulo Augusto Raymundo Pereira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/18270-2 - Development of biosensors based on zein, hemoglobin and carbon black for hydrogen peroxide detection
• Grantee: Tamires dos Santos Pereira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/19099-2 - Development of electrochemical sensors and biosensors for different analytical purposes
• Grantee: Bruno Campos Janegitz
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/04743-6 - Electrode carbon vitreous modified with titanium dioxide nanotubes and tapioca film for detection of hormone estradiol
• Grantee: Gabriela Carolina Mauruto de Oliveira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation