Interfacial vibrational spectroscopy and Brewster angle microscopy distinguishing the interaction of terpineol in cell membrane models at the air-water interface

Author(s)

Jaroque, Guilherme Nunez ^[1] ; Sartorelli, Patricia ^[1] ; Caseli, Luciano ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Inst Envinonmental Chem & Pharmaceut Sci, Diadema, SP - Brazil

• Document type: Journal article
• Source: Biophysical Chemistry; v. 246, p. 1-7, MAR 2019.
• Web of Science Citations: 1

Abstract

In this paper, gamma-terpineol, a known monoterpene with biological activity, including in lipidic interfaces, was incorporated in Langmuir monolayers of selected phospholipids as a model for cellular membranes. Surface pressure-area isotherms showed that selected amounts of gamma-terpineol expand DPPC and DPPE monolayers and decreased the monolayer elasticity, confirming the lipid/compound interaction. Characterization with vibrational spectroscopy and Brewster angle microscopy pointed that gamma-terpineol adsorbs on the polar heads of the phospholipids, affecting the gauche conformations of the aliphatic chains, and different patterns were observed for DPPC and DPPE monolayers, indicating a characteristic molecular accommodation of gamma-terpineol along the polar head of the phospholipids. Therefore, distinctive interactions with DPPC and DPPE could be observed regarding the incorporation of gamma-terpineol with each lipid, leading to particular molecular arrangements at the air-water interface and pointing the modulation of the interaction according to the chemical composition of the monolayer. (AU)

• FAPESP's process: 15/23446-0 - Smart surfaces: nanostructured and bioinspired systems for the investigation of molecular interactions in membrane models and for the production of devices for biosensors and environmental analysis
• Grantee: Luciano Caseli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants