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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phenotypic, molecular and genomic characterization of Actinobaculum suis isolated from swine in Brazil

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Author(s):
Amigo, Cristina Roman [1] ; Moreno, Luisa Zanolli [1, 2] ; Porfida Ferreira, Thais Sebastiana [1] ; de Moura Gomes, Vasco Tulio [1] ; Poor, Andre Pegoraro [1] ; de Oliveira, Carolina Helena [1] ; Parra, Beatriz Martins [1] ; Santos Silva, Ana Paula [1] ; Moreno, Andrea Micke [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, Sao Paulo - Brazil
[2] Ctr Univ Max Planck UniMax, Indaiatuba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Anaerobe; v. 56, p. 27-33, APR 2019.
Web of Science Citations: 0
Abstract

Urinary tract infections (UTI) are considered one of the most important diseases of sows due to its close relationship with reproductive problems such as reduced litter size, increase in the rate of return to estrous, vulvar discharge, abortion, mastitis and anestrus. Actinobaculum suis is one of the main agents involved in porcine urinary tract infection and is responsible for the most severe and fatal cases in sows. In the present report, 23 A. suis strains isolated from a sow and boars in Brazil were identified by PCR and further characterized by broth microdilution, molecular typing by pulsed-field gel electrophoresis (PFGE), single-enzyme amplified fragment length polymorphism (SE-AFLP), and whole-genome sequencing. All strains were sensitive to ceftiofur, linezolid, nitrofurantoin, quinupristin-dalfopristin and vancomycin. Ciprofioxacin, daptomycin, lincomycin, erythromycin and tylosin resistance was observed in 100% of tested strains. Tetracycline and tigecycline also presented high resistance rates (87% and 30.4%, respectively). PFGE with eight different restriction enzymes and three programs did not enable strain characterization; however, all strains were typed by SE-AFLP that clustered strains according to their origin, thus proving an effective tool for A. suis genotyping. Whole-genome sequencing and comparative analysis enabled species differentiation from closely related genus. This is the first report of genomic characterization of A. suis. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 17/09515-4 - Identification and phenotypic characterization of bacterial pathogens causing metritis in sows.
Grantee:André Pegoraro Poor
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/25745-7 - Virulence assessment and phenotypic and genomic characterization of Corynebacterium spp. isolated from sow's urinary tract infection
Grantee:Luisa Zanolli Moreno
Support type: Scholarships in Brazil - Post-Doctorate