A first step towards building spectral libraries as complementary tools for snake venom proteome/peptidome studies

Author(s)

Zelanis, Andre ^[1] ; Silva, Debora A. ^[2] ; Kitano, Eduardo S. ^{[2, 3]} ; Liberato, Tarcisio ^[1] ; Fukushima, Isabella ^[1] ; Serrano, Solange M. T. ^[2] ; Tashima, Alexandre K. ^{[2, 4]}

Affiliation

^[1] Univ Fed Sao Paulo ICT UNIFESP, Dept Sci & Technol, Funct Prote Lab, Sao Jose Dos Campos, SP - Brazil

^[2] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Lab Especial Toxinol Aplicada, Sao Paulo - Brazil

^[3] Univ Sao Paulo HCFMUSP, Fac Med, Hosp Clin, Lab Imunol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo EPM UNIFESP, Escola Paulista Med, Dept Bioquim, Sao Paulo - Brazil

• Document type: Journal article
• Source: Comparative Biochemistry and Physiology D-Genomics & Proteomics; v. 31, SEP 2019.
• Web of Science Citations: 0

Abstract

Snake venoms are complex mixtures of a large number of distinct proteins and peptides with biological activity. Peptide spectral libraries are compilations of previously identified MS/MS spectra obtained from proteomics experiments. Here we present the generation and use of a Venom Peptidome and a Venom Proteome spectral library for the analysis of venom proteomes and peptidomes from distinct snake species. (AU)

• FAPESP's process: 13/14651-3 - Experimental approaches in proteomics and glycomics applied to the characterization of Bothrops alcatraz
• Grantee: Débora Andrade Silva
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/06579-3 - System-wide analysis of N-terminal processing and protein diversity in the secretome of tumor cells
• Grantee: André Zelanis Palitot Pereira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 17/20106-9 - Peptidomics of Brazilian snake and spider venoms
• Grantee: Alexandre Keiji Tashima
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
• Grantee: Hugo Aguirre Armelin
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 17/07897-7 - Proteolytic processing analysis in the secretome of melanoma tumor cells
• Grantee: Isabella Fukushima
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/13548-4 - Proteomic/Glycoproteomic characterization of the venoms of the Bothrops jararaca complex with emphasis on the N-terminome and N-glycome of toxins
• Grantee: Solange Maria de Toledo Serrano
• Support Opportunities: Regular Research Grants