Natural deep eutectic solvents and aqueous solutions as an alternative extraction media for propolis

Author(s)

Funari, Cristiano Soleo ; Sutton, Adam T. ^[1] ; Carneiro, Renato Lajarim ^[2] ; Fraige, Karina ^[3] ; Cavalheiro, Alberto Jose ^[3] ; Bolzani, Vanderlan da Silva ^[3] ; Hilder, Emily F. ^[1] ; Dario Arrua, R. ^[1]

Affiliation

^[1] Univ South Australia UniSA, Future Ind Inst, Adelaide, SA - Australia

^[2] Fed Univ Sao Carlos UFSCar, Dept Chem, Sao Carlos, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil

• Document type: Journal article
• Source: Food Research International; v. 125, NOV 2019.
• Web of Science Citations: 1

Abstract

Ethanolic extracts of propolis are consumed for their health benefits even though direct consumption of alcoholic extracts is not always ideal. Natural Deep Eutectic Solvents (NADES) can potentially extract similar compounds as alcoholic extracts while being better for direct consumption. Therefore, in this work alternative solvents for the extraction of green propolis including its biomarker artepillin C were examined. Sixteen NADES made from low toxicity chemicals, including the essential amino acid L-lysine, were explored along with twelve individual NADES components and honey, which showed similar physical-chemical properties to NADES. At 50 degrees C NADES made from choline chloride-propylene glycol or lactic acid proved to be equal or better than the benchmark EtOH:Water 7:3 (v/v). Alternatively, aqueous t.-lysine appeared as a potential solvent for the preparation of aqueous propolis extracts. From these findings NADES, honey and aqueous L-lysine solutions all demonstrated the potential to replace ethanol or water for extracting green propolis. (AU)

• FAPESP's process: 17/06216-6 - Development of green analytical methodologies in the search for high-added value organic compounds in agricultural waste
• Grantee: Cristiano Soleo de Funari
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 13/15086-8 - Application of metabolomics and proteomics in searching for new biologically active compounds in Malpighiaceae plant species
• Grantee: Karina Fraige Baraco
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 16/50009-2 - Greening sample preparation of complex matrices by using natural deep eutectic solvents (NADES) together with porous polymeric adsorbents for subsequent analyses by liquid chromatography (LC) and nuclear magnetic resonance (NMR)
• Grantee: Vanderlan da Silva Bolzani
• Support Opportunities: Regular Research Grants