Non-enzymatic electrochemical determination of creatinine using a novel screen-printed microcell

Author(s)

Fava, Elson Luiz ^[1] ; do Prado, Thiago Martimiano ^[1] ; Garcia-Filho, Amauri ^[1] ; Silva, Tiago Almeida ^{[1, 2]} ; Cincotto, Fernando Henrique ^{[3, 1]} ; de Moraes, Fernando Cruz ^[1] ; Faria, Ronaldo Censi ^[1] ; Fatibello-Filho, Orlando ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Chem, POB 676, BR-13560970 Sao Carlos, SP - Brazil

^[2] Fed Ctr Technol Educ Minas Gerais, Dept Met & Chem, BR-35180008 Timoteo, MG - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Chem, Rio De Janeiro, RJ - Brazil

• Document type: Journal article
• Source: Talanta; v. 207, JAN 15 2020.
• Web of Science Citations: 0

Abstract

A low-cost and disposable microcell was constructed with a screen-printed electrode for the non-enzymatic electrochemical determination of creatinine. The working electrode was modified with carbon black and maintained in contact with paper-adsorbed iron (III) ions. A small sample volume of 3 mu L was required for the device operation. Then, iron (III) ions were complexed in the presence of creatinine in a chemical step, followed by an electrochemical reduction of non-complexed metallic ions in excess. Cyclic voltammetry and differential-pulse voltammetry experiments were employed for the electrochemical characterizations and analytical performance evaluation of the microcell. The working electrode modification with carbon black provided a significant increase of analytical signal. The sensor presented a linear response for creatinine concentrations ranging from 0.10 to 6.5 mmol L-1, with a limit of detection of 0.043 mmol L-1. Experiments for creatinine determination in real samples were successful performed through of standard recovery in urine. (AU)

• FAPESP's process: 17/24053-7 - Development of simple electrochemical methods for the detection of biomarkers for the diagnosis of diseases
• Grantee: Ronaldo Censi Faria
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/10012-4 - Development of a disposable biosensors array with a microfluidic device for multiplex determination of species of biological interest in different matrices
• Grantee: Elson Luiz Fava
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/10118-0 - Study and application of electrochemical technology for the analysis and degradation of endocrine interferents: materials, sensors, processes and scientific dissemination
• Grantee: Marcos Roberto de Vasconcelos Lanza
• Support Opportunities: Research Projects - Thematic Grants