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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular eco-epidemiology on the sympatric Chagas disease vectors Triatoma brasiliensis and Triatoma petrocchiae: Ecotopes, genetic variation, natural infection prevalence by trypanosomatids and parasite genotyping

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Lima-Oliveira, Tarcianne M. [1, 2] ; Fontes, Fernanda von Hertwig M. [2] ; Lilioso, Mauricio [2] ; Pires-Silva, Dayane [2] ; Geraldes Teixeira, Marta Maria [3] ; Vergara Meza, Jose Gabriel [3] ; Harry, Myriam [4] ; Filee, Jonathan [4] ; Costa, Jane [5] ; Valenca-Barbosa, Carolina [2] ; Folly-Ramos, Elaine [1] ; Almeida, Carlos Eduardo [2]
Total Authors: 12
Affiliation:
[1] Univ Fed Paraiba UFPB, Programa Posgrad Ecol & Monitoramento Ambiental, Campus 4, BR-58297 Rio Tinto, Paraiba - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Biol Anim, UNICAMP, BR-13083862 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Lab Taxon & Filogenia Tripanossomatideos, BR-05508000 Sao Paulo, SP - Brazil
[4] Univ Paris Saclay, Univ Paris Sud, IDEEV, CNRS, IRD, F-91190 Gif Sur Yvette - France
[5] Fundacao Oswaldo Cruz Fiocruz, Lab Biodivers Entomol, Inst Oswaldo Cruz, BR-21040900 Rio De Janeiro, RJ - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Acta Tropica; v. 201, JAN 2020.
Web of Science Citations: 0
Abstract

Triatoma petrocchiae is the newly member of the Triatoma brasiliensis species complex. This species overlaps with T. brasiliensis in geographic and ecotypic occupation in the sylvatic habitat because both inhabit rocky outcrops in the semi-arid portion of Brazilian northeast. In this region T. brasiliensis is the most important Chagas disease vector because it constantly colonizes domiciles. In contrast, T. petrocchiae is rarely found in peri or intradomiciliary habitats - reason why little is known about this species. Therefore, Here, we present information for the first time on. the T. petrocchiae ecotopes, genetic diversity, Trypanosoma cruzi prevalence/genotyping in comparison to T. brasiliensis. We found T. brasilensis (N=223) and T. petrocchiae (N=69) in co-habitation in rocky outcrops in three Districts of Paraiba and Rio Grande do Norte states. Forty-tree T. petrocchiae insects of eleven sampling spots (composing three geographic populations) were genotyped for the mitochondrial Cyt B gene and little geographic structure was observed. Tajima's D test suggested that species is evolving toward a mutation-drift equilibrium in our collection range. Sylvatic T. petrocchiae had 4% (3/68) of infected insects by T. cruzi, whereas T. brasiliensis had 26% (59/223). Fluorescent Fragment Length Barcoding demonstrated that all three T. petrocchiae harbored TcI whereas T. brasiliensis had TcI, but also TcIII, TcII/TcVI and T. rangeli genotype A, sometimes under mixed infections. None of infected T. petrocchiae were carrying mixed infections. However, this result should be confirmed using a larger pool of infected bugs. We here presented the first documentation of T. rangeli infecting T. brasiliensis. The finding of infected T. petrocchiae calls for constant vector monitoring because the epidemiologic scenario is dynamic and sylvatic vectors are progressively found in adaptation to anthropic environments. (AU)

FAPESP's process: 17/50329-0 - Bilateral cooperation (UNICAMP-CNRS) to optimize ongoing studies on molecular eco-epidemiology on vectors
Grantee:Carlos Eduardo de Almeida
Support Opportunities: Regular Research Grants
FAPESP's process: 16/08176-9 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas Disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain
Grantee:Carlos Eduardo de Almeida
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 17/21359-8 - Integrated molecular approaches to elucidate the ecological associations of Triatoma brasiliensis: food sources, microbiota and diversity of Trypanosoma cruzi
Grantee:Maurício Lilioso de Lucena Filho
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/09088-9 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain
Grantee:Carlos Eduardo de Almeida
Support Opportunities: Scholarships in Brazil - Young Researchers