Dietary fiber and fiber-bound polyphenols of grape peel powder promote GSH recycling and prevent apoptosis in the colon of rats with TNBS-induced colitis

Author(s)

Maurer, Luana Haselein ^{[1, 2]} ; Betim Cazarin, Cinthia Bau ^[3] ; Quatrin, Andreia ^{[1, 4]} ; Nichelle, Sabrina Marafiga ^[4] ; Minuzzi, Natalia Machado ^[4] ; Teixeira, Cibele Ferreira ^[5] ; Manica da Cruz, Ivana Beatrice ^[5] ; Marostica Junior, Mario Roberto ^[3] ; Emanuelli, Tatiana ^{[1, 4]}

Affiliation

^[1] Univ Fed Santa Maria, Ctr Rural Sci, Grad Program Food Sci & Technol, BR-97105900 Santa Maria, RS - Brazil

^[2] Fed Inst Educ Sci & Technol Farroupilha, BR-97555000 Alegrete, RS - Brazil

^[3] Univ Estadual Campinas, Sch Food Engn, Dept Food & Nutr, BR-13083862 Campinas, SP - Brazil

^[4] Univ Fed Santa Maria, Ctr Rural Sci, Integrated Ctr Lab Anal Dev NIDAL, Dept Food Technol & Sci, BR-97105900 Santa Maria, RS - Brazil

^[5] Univ Fed Santa Maria, Grad Program Pharmacol, BR-97105900 Santa Maria, RS - Brazil

• Document type: Journal article
• Source: Journal of Functional Foods; v. 64, JAN 2020.
• Web of Science Citations: 0

Abstract

Ulcerative colitis (UC) is a chronic inflammatory bowel disease that reduces glutathione (GSH) levels. We aimed to investigate the effect of dietary supplementation with grape peel powder (GPP) on the GSH system in a rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to identify the role of grape bioactive fractions, namely soluble phenolics (EP), fiber-bound polyphenols (NEP-F), and dietary fiber (F). Colitis reduced colonic GSH levels and glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) activities. NEP-F completely restored GR and GPx, while all diets restored GST activities. Colitis increased the mRNA expression of both subunits of glutamate-cysteine ligase and induced apoptosis in the colon. The reestablishment of GSH levels promoted by GPP was associated with the recycling of oxidized glutathione by increased GR activity instead of GSH synthesis. NEP-F and F fractions were responsible for the protective effect of GPP against apoptosis. (AU)

• FAPESP's process: 15/50333-1 - COBRA: a novel compound discovery pipeline for rapid and cost-effective identification of bioactives with health beneficial effects in Brazilian crops
• Grantee: Glaucia Maria Pastore
• Support Opportunities: Research Projects - Thematic Grants