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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Simple and inexpensive electrochemical paper-based analytical device for sensitive detection of Pseudomonas aeruginosa

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Author(s):
Ferreira e Silva, Rafael [1] ; Longo Cesar Paixao, Thiago Regis [1] ; Torossian Torres, Marcelo Der [2, 3, 4] ; de Araujo, William Reis [5]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil
[3] Univ Penn, Inst Translat Med & Therapeut, Perelman Sch Med, Inst Biomed Informat, Dept Bioengn, Machine Biol Grp, Dept Psychiat, Philadelphia, PA 19104 - USA
[4] Univ Penn, Inst Translat Med & Therapeut, Perelman Sch Med, Inst Biomed Informat, Dept Bioengn, Machine Biol Grp, Dept Microbiol, Philadelphia, PA 19104 - USA
[5] Univ Estadual Campinas, UNICAMP, Dept Analyt Chem, Inst Chem, POB 6154, BR-13083970 Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: SENSORS AND ACTUATORS B-CHEMICAL; v. 308, APR 1 2020.
Web of Science Citations: 0
Abstract

Pseudomonas aeruginosa (PA) is an opportunistic pathogen responsible for several diseases in humans and it is one of the main causes of hospital-acquired infections exhibiting a high drug-resistance profile. Hence, the rapid detection of pathogenic infections caused by bacteria in biofluids from patients or screening contaminated surfaces/utensils is of the utmost importance to healthcare, especially in places with high clinical demand and in resource-limited settings. Herein, we report a portable, selective, and very sensitive method (ranging nmol L-1 concentrations) for PA detection in human saliva and contaminated surfaces, without any sample preparation or separation steps. We used an inexpensive and disposable electrochemical paper-based analytical device (ePAD) for detecting pyocyanin (PYO), a key biomarker virulence factor that is unique for this pathogen. Additionally, we followed PA growth of two virulent strains, PA14 and PAO1, and found a high correlation between the electrochemically detected PYO levels and the optical density values of PA colony forming units (CFU), demonstrating the usefulness of the device for monitoring the bacterial growth and the early detection of PA infection in several relevant applications. (AU)

FAPESP's process: 17/11398-6 - Development of portable electrochemical sensor in paper platform for detection of bacterial infection by Pseudomonas aeruginosa in biological fluids
Grantee:Rafael Ferreira e Silva
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/10522-5 - Development of alternative methods for fabrication of electrochemical paper-based sensors
Grantee:Thiago Regis Longo Cesar da Paixão
Support Opportunities: Regular Research Grants
FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
Grantee:Mauro Bertotti
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/16477-9 - Fabrication of portable paper-based analytical devices with electrochemical and colorimetric detection system aiming at forensic, clinical, and environmental applications
Grantee:William Reis de Araujo
Support Opportunities: Scholarships in Brazil - Post-Doctoral