S-nitrosothiol-terminated poly(vinyl alcohol): Nitric oxide release and skin blood flow response

Author(s)

Giglio, Leonardo P. ^[1] ; Picheth, Guilherme F. ^[1] ; Lovschall, Kaja Borup ^[2] ; Zelikin, Alexander N. ^{[2, 3]} ; de Oliveira, Marcelo G. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Unicamp, Inst Chem, BR-13083861 Campinas, SP - Brazil

^[2] Aarhus Univ, Dept Chem, DK-8000 Aarhus C - Denmark

^[3] Aarhus Univ, INano Interdisciplinary Nomsci Ctr, DK-8000 Aarhus C - Denmark

• Document type: Journal article
• Source: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 98, p. 41-49, MAY 1 2020.
• Web of Science Citations: 0

Abstract

Polymeric biomaterials capable of delivering nitric oxide (NO) topically can be used to enhance skin blood flow (SkBF) and accelerate wound healing. Herein, we used reversible addition-fragmentation chain transfer radical (RAFT) polymerization to synthesize the first poly(vinyl alcohol) (PVA) functionalized with terminal NO-releasing S-nitrosothiol (RSNO) groups for topical NO delivery. This strategy was based on the synthesis of a precursor amino-terminated PVA (PVA-NH2), which was next functionalized with iminothiolane yielding 4-imino-4-amino-PVA-butane-1-thiol (PVA-SH), and finally S-nitrosated yielding S-nitroso 4-imino-4-amino-PVAbutane-1-thiol (PVA-SNO). Real-time chemiluminescence NO detection showed that blended films of pure PVA with PVA-SNO with mass ratios 30:70, 50:50 and 70:30 release NO with initial rates ranging from 1 to 12 nmol g(-1) min(-1), and lead to a 2 to 10-fold dose-response increase in the SkBF, after topical application on the ventral forearm of volunteers. These results show that PVA-SNO is a potential platform for topical NO delivery in biomedical applications. (AU)

• FAPESP's process: 17/04622-7 - Electrospun PVA-GSNO fibers: preparation and biological activity
• Grantee: Leonardo Pinheiro Giglio
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• FAPESP's process: 16/02414-5 - Topical and absorbable biomaterials for the local nitric oxide release
• Grantee: Marcelo Ganzarolli de Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/19253-7 - Pluronic F127 thermosensitive hydrogels functionalized with terminal groups releasing nitric oxide
• Grantee: Guilherme Fadel Picheth
• Support Opportunities: Scholarships in Brazil - Post-Doctoral