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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Wound healing action of nitric oxide-releasing self-expandable collagen sponge

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Povoa, Valeria C. O. [1] ; dos Santos, Giovanna J. V. P. [2] ; Picheth, Guilherme F. [2] ; Jara, Carlos P. [1] ; da Silva, Laura C. E. [2] ; de Araujo, Eliana P. [1] ; de Oliveira, Marcelo G. [2]
Total Authors: 7
[1] Univ Estadual Campinas, Nursing Sch, UNICAMP, Campinas 13084970, SP - Brazil
[2] Univ Estadual Campinas, Inst Chem, UNICAMP, Campinas 13083970, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 0

Mounting evidence showing that local nitric oxide (NO) delivery may significantly improve the wound healing process has stimulated the development of wound dressings capable of releasing NO topically. Herein, we describe the preparation of a self-expandable NO-releasing hydrolyzed collagen sponge (CS), charged with the endogenously found NO donor, S-nitrosoglutathione (GSNO). We show that cold pressed and GSNO-charged CS (CS/GSNO) undergo self-expansion to its original 3D shape upon water absorption to a swelling degree of 2,300 wt%, triggering the release of free NO. Topical application of compressed CS/GSNO on wounds in an animal model showed that exudate absorption by CS/GSNO leads to the release of higher NO doses during the inflammatory phase and progressively lower NO doses at later stages of the healing process. Moreover, treated animals showed significant increase in the mRNA expression levels of monocyte chemoattractant protein-1 (MCP-1), murine macrophage marker (F4/80), transforming growth factor beta (TGF-beta), stromal cell-derived factor 1 (SDF-1), insulin-like growth factor-1 (IGF-1), nitric oxide synthase(iNOS), and matrix metalloproteinase(MMP-9). Cluster differentiation 31 (CD31), vascular endothelial growth factor (VEGF), and F4/80 were measured on Days 7 and 12 by immunohistochemistry in the cicatricial tissue. These results indicate that the topical delivery of NO enhances the migration and infiltration of leucocytes, macrophages, and keratinocytes to the wounded tissue, as well as the neovascularization and collagen deposition, which are correlated with an accelerated wound closure. Thus, self-expandable CS/GSNO may represent a novel biocompatible and active wound dress for the topical delivery of NO on wounds. (AU)

FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
Grantee:Licio Augusto Velloso
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 18/14142-5 - Hybrid hydrogels for local and prolonged nitric oxide release
Grantee:Laura Caetano Escobar da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/04615-0 - Nitric oxide releasing collagen membranes for bone regeneration
Grantee:Giovanna Jardim Vieira Pereira dos Santos
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/02414-5 - Topical and absorbable biomaterials for the local nitric oxide release
Grantee:Marcelo Ganzarolli de Oliveira
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/19253-7 - Pluronic F127 thermosensitive hydrogels functionalized with terminal groups releasing nitric oxide
Grantee:Guilherme Fadel Picheth
Support type: Scholarships in Brazil - Post-Doctorate