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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

BACTERIAL NANOCELLULOSE BIOMEMBRANE AS A SUPPORT FOR HUMAN INSULIN AIMING AT TRANSDERMAL PERMEATION

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Author(s):
Ludmilla R. Jorge [1] ; Liliam K. Harada [2] ; Erica C. Silva [3] ; Welida F. Campos [4] ; José M. Oliveira Jr. [5] ; Marta M. D. C. Vila [6] ; Matthieu Tubino [7] ; Victor M. Balcão
Total Authors: 8
Affiliation:
[1] Universidade de Sorocaba. PhageLab - Laboratório de Biofilmes e Bacteriófagos - Brasil
[2] Universidade de Sorocaba. PhageLab - Laboratório de Biofilmes e Bacteriófagos - Brasil
[3] Universidade de Sorocaba. PhageLab - Laboratório de Biofilmes e Bacteriófagos - Brasil
[4] Universidade de Sorocaba. PhageLab - Laboratório de Biofilmes e Bacteriófagos - Brasil
[5] Universidade de Sorocaba. PhageLab - Laboratório de Biofilmes e Bacteriófagos - Brasil
[6] Universidade de Sorocaba. PhageLab - Laboratório de Biofilmes e Bacteriófagos - Brasil
[7] Universidade Estadual de Campinas. Instituto de Química - Brasil
Total Affiliations: 8
Document type: Journal article
Source: Química Nova; v. 43, n. 5, p. 572-578, 2020-06-29.
Abstract

Production of bacterial nanocellulose was pursued as a matrix system for the stabilization of human insulin. The biomembranes produced by Gluconacetobacter hansenii were washed with 2% aqueous sodium dodecylsulfate solution, rinsed with ultrapure water and immersed in 1 mol L-1 NaOH aqueous solution at 60 °C for 90 min until neutralization. For the insulin adsorption assays, the biomembranes were soaked in a buffered solution of human insulin until no protein could be detected in the supernatant. The membranes with adsorbed insulin were characterized via mechanical resistance (resilience, relaxation, perforation), Differential Scanning Calorimetry (DSC), Thermal Gravimetrical Analysis (TGA), Fourier Transform Infrared Spectrophotometry (FTIR), X-ray diffraction (XRD) and Field Emission Scanning Electron Microscopy (FESEM) analyses. The FESEM photomicrographs of the surface of the biomembranes showed a rugged surface without cracks. The biomembranes exhibited adequate mechanical characteristics. The infrared spectra indicated that the chemical aspect of the protein moiety was preserved during adsorption onto the BNC biomembranes. According to the XRD analyses, the biomembranes showed a generalized amorphous behavior. Thermal analyses indicated an adequate thermal stability for a pharmaceuticals product. Hence, an elastic and malleable biomembrane was produced, suitable for incorporation of human insulin, aiming at transdermal delivery. (AU)

FAPESP's process: 16/12234-4 - TransAppIL - Transdermal delivery of structurally and functionally stabilized protein entities applying ionic liquids.
Grantee:Marta Maria Duarte Carvalho Vila
Support Opportunities: Regular Research Grants
FAPESP's process: 18/05522-9 - PsaPhageKill - isolation, characterisation and use of lytic bacteriophages against Pseudomonas syringae PV. actinidiae to fight the cancer of kiwifruit: an efficient and ecofriendly alternative
Grantee:Vitor Manuel Cardoso Figueiredo Balcão
Support Opportunities: Scholarships abroad - Research
FAPESP's process: 16/16641-3 - Structural and functional stabilization of sericin in a biopolysaccharide hydrogel: bio-origami for skin regeneration
Grantee:Liliam Katsue Harada Rocha
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 16/08884-3 - PneumoPhageColor - development of a colorimetric biodetection kit for Pseudomonas aeruginosa based on phage particles
Grantee:Vitor Manuel Cardoso Figueiredo Balcão
Support Opportunities: Regular Research Grants