Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Automated needle-sleeve based online hyphenation of solid-phase microextraction and liquid chromatography

Full text
da Silva, Luis Felipe [1] ; Vargas Medina, Deyber Arley [1] ; Lancas, Fernando Mauro [1]
Total Authors: 3
[1] Univ Sao Paulo, Inst Chem Sao Carlos, Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Talanta; v. 221, JAN 1 2021.
Web of Science Citations: 0

A novel approach for the online coupling of solid-phase micmextraction (SPME) and liquid chromatography (LC) is introduced. An innovative Si@GO@beta CD coated needle-sleeve extractant device was developed and then employed in the automated online SPME-LC-UV determination of estrogen-like isoflavones from human urine samples. The extractant SPME device is easily attachable at the endpoint of an analytical syringe needle and operated by a lab-made autosampler. Fully automated online SPME-LC is accomplished by proper autosampler programming to perform the following steps: i) the analytes extraction by direct immersion of the extractant device into the stirred sample, ii) a rinsing step iii) the analytes desorption/enrichment, iv) the online transference of the extract to the LC injection valve. Besides allowing the online SPME hyphenation, this extraction modality efficiently addressed the drawbacks associated with the clogging and dispersion of graphene-based microextraction techniques performed in packed-bed and dispersive formats. The main extraction parameters and the performance of the automated online SPME-LC method developed were carefully studied. The results show a good sensitivity, reliability, and straightforward analytical strategy for the determination of organic compounds in complex samples. The detection limit of the method was 20 mu g L-1 for DAI and 10 mu g L-1 for GEN, FOR and BIO. The intra-day RSD was below 10% and inter-day RSD was below 13%. The total analysis time was less than 17 min per sample. (AU)

FAPESP's process: 14/07347-9 - Development of novel materials and devices for miniaturized sample preparation techniques in the area of food safety
Grantee:Fernando Mauro Lanças
Support type: Regular Research Grants
FAPESP's process: 14/03765-0 - Development of an automated solid-phase microextraction based on an arrangement with microsyringes packed sorbent for the simultaneous preparation of multiple samples
Grantee:Deyber Arley Vargas Medina
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/15462-5 - Employment of graphene as sorbent in miniaturized sample preparation techniques.
Grantee:Bruno Henrique Fumes
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
Grantee:Fernando Mauro Lanças
Support type: Research Projects - Thematic Grants
FAPESP's process: 19/22724-7 - Novel instrumental approaches for automated and miniaturized analytical methods for the detection of organic compounds by liquid chromatography coupled to mass spectrometry
Grantee:Deyber Arley Vargas Medina
Support type: Scholarships in Brazil - Post-Doctorate