Phenolic profile and potential beneficial effects of underutilized Brazilian native fruits on scavenging of ROS and RNS and anti-inflammatory and antimicrobial properties

Author(s)

Soares, Jackeline Cintra ^[1] ; Rosalen, Pedro Luiz ^{[2, 3]} ; Lazarini, Josy Goldoni ^[2] ; Orlandi Sardi, Janaina de Cassia ^[4] ; Massarioli, Adna Prado ^[1] ; Nani, Bruno Dias ^[2] ; Franchin, Marcelo ^{[2, 5]} ; de Alencar, Severino Matias ^[1]

Affiliation

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind, Ave Padua Dias, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Dept Physiol Sci, Piracicaba Dent Sch, Ave Limeira 901, BR-13414018 Piracicaba, SP - Brazil

^[3] Univ Fed Alfenas, Grad Program Biol Sci, Alfenas, MG - Brazil

^[4] Univ Fed Mato Grosso, Sch Pharmaceut Sci Food & Nutr, Campo Grande, MS - Brazil

^[5] Fed Univ Alfenas MG, Sch Dent, Unifal MG, Alfenas - Brazil

• Document type: Journal article
• Source: FOOD & FUNCTION; v. 11, n. 10, p. 8905-8917, OCT 1 2020.
• Web of Science Citations: 0

Abstract

Brazilian native fruits (BNF) have aroused interest of researchers and consumers for their great human health benefits. In this study, five BNF (Byrsonima lancifolia, Campomanesia phaea, Jacaratia spinosa, Solanum alternatopinnatum and Acnistus arborescens) were tested for their polyphenolic compounds by LC-ESI-MS/MS, reactive species deactivation (ROO\_, O2\_-, HOCl and NO\_), anti-inflammatory properties in vivo, and in vitro antimicrobial activity - with determination of putative mechanism(s) of action. Eightyone polyphenols were identified, which exhibited a significant capacity to deactivate both ROS and RNS. C. phaea extract had the highest capacity to scavenge ROO\_ (68.94 mu mol TE per g), O2\_- (IC50: 575.36 mu g mL-1) and NO\_ (IC50: 16.96 mu g mL-1), which may be attributed to the presence of ellagitanins. B. lancifolia decreased neutrophil influx into the peritoneal cavity of mice by 50% as compared to carrageenan and reduced Candida albicans biofilm viability by 3 log10 possibly due to complexation with cell membrane ergosterol. In summary, the BNF presented herein are good sources of bioactive compounds with positive effects on deactivation of biological reactive species, as well as with anti-inflammatory and antimicrobial activities, which can be altogether highly beneficial to human health. (AU)

• FAPESP's process: 17/09898-0 - Bioprospection of novel anti-inflammatory molecules from natural Brazilian native products
• Grantee: Pedro Luiz Rosalen
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/02926-6 - Phytochemistry and bioprospection of antioxidant and anti-inflammatory activities from unexplored Brasilian native fruits.
• Grantee: Josy Goldoni Lazarini
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/13190-2 - Phenolic composition and biological potential, in vitro and in vivo, from native fruits of Brazil
• Grantee: Severino Matias de Alencar
• Support Opportunities: Regular Research Grants