Microbiota-derived short-chain fatty acids do not interfere with SARS-CoV-2 infection of human colonic samples

Author(s):Show less -

Pascoal, Livia Bitencourt ^{[1, 2]} ; Rodrigues, Patricia Brito ^[1] ; Genaro, Livia Moreira ^[2] ; dos Santos Pereira Gomes, Arilson Bernardo ^[1] ; Toledo-Teixeira, Daniel Augusto ^[3] ; Parise, Pierina Lorencini ^[3] ; Bispo-Dos-Santos, Karina ^[3] ; Simeoni, Camila Lopes ^[3] ; Guimaraes, Paula Veri ^[2] ; Buscaratti, Lucas Ildefonso ^[4] ; De Angeli Elston, Joao Gabriel ^[4] ; Marques-Souza, Henrique ^[4] ; Martins-de-Souza, Daniel ^{[5, 6]} ; Setsuko Ayrizono, Maria De Lourdes ^[2] ; Velloso, Licio Augusto ^{[7, 8]} ; Proenca-Modena, Jose Luiz ^{[6, 3]} ; Mendes Moraes-Vieira, Pedro Manoel ^{[6, 9]} ; Silva Mori, Marcelo Alves ^{[6, 10]} ; Farias, Alessandro Santos ^{[6, 11]} ; Ramirez Vinolo, Marco Aurelio ^{[1, 6]} ; Leal, Raquel Franco ^{[2, 7]}

Affiliation:Show less -

^[1] Univ Estadual Campinas, UNICAMP, Dept Genet Microbiol & Immunol, Lab Immunoinflammat, Inst Biol, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Sch Med Sci, Dept Surg, Inflammatory Bowel Dis Res Lab, Colorectal Surg Un, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Dept Genet Microbiol & Immunol, Lab Emerging Viruses, Inst Biol, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, UNICAMP, Dept Biochem & Tissue Biol, Brazilian Lab Silencing Technol Blast, Inst Biol, Campinas - Brazil

^[5] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Lab Neuroprote, UNICAMP, Campinas, SP - Brazil

^[6] Univ Estadual Campinas, UNICAMP, Expt Med Res Cluster EMRC, Campinas - Brazil

^[7] Univ Estadual Campinas, UNICAMP, Obes & Comorbid Res Ctr OCRC, Campinas - Brazil

^[8] Univ Estadual Campinas, Sch Med Sci, Lab Cell Signaling, UNICAMP, Campinas, SP - Brazil

^[9] Univ Estadual Campinas, Inst Biol, Dept Genet Microbiol & Immunol, Lab Immunometab, UNICAMP, Campinas, SP - Brazil

^[10] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Lab Aging Biol, UNICAMP, Campinas, SP - Brazil

^[11] Univ Estadual Campinas, Inst Biol, Dept Genet Microbiol & Immunol, Autoimmune Res Lab, UNICAMP, Campinas, SP - Brazil

• Document type: Journal article
• Source: GUT MICROBES; v. 13, n. 1, p. 1-9, JAN 1 2021.
• Web of Science Citations: 9

Abstract

Microbiota-derived molecules called short-chain fatty acids (SCFAs) play a key role in the maintenance of the intestinal barrier and regulation of immune response during infectious conditions. Recent reports indicate that SARS-CoV-2 infection changes microbiota and SCFAs production. However, the relevance of this effect is unknown. In this study, we used human intestinal biopsies and intestinal epithelial cells to investigate the impact of SCFAs in the infection by SARS-CoV-2. SCFAs did not change the entry or replication of SARS-CoV-2 in intestinal cells. These metabolites had no effect on intestinal cells' permeability and presented only minor effects on the production of anti-viral and inflammatory mediators. Together our findings indicate that the changes in microbiota composition of patients with COVID-19 and, particularly, of SCFAs do not interfere with the SARS-CoV-2 infection in the intestine. (AU)

• FAPESP's process: 20/04746-0 - Understanding the molecular bases and the role of risk factors associated to SARS-CoV-2 infection in preclinical models
• Grantee: Daniel Martins-de-Souza
• Support type: Regular Research Grants
• FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
• Grantee: Licio Augusto Velloso
• Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 20/02312-3 - Study of the effect of short-chain fatty acids on the tissue regeneration process during experimental colitis
• Grantee: Arilson Bernardo dos Santos Pereira Gomes
• Support type: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 20/04919-2 - Determining molecular candidates that contribute to the higher risk of COVID-19 in aged individuals
• Grantee: Marcelo Alves da Silva Mori
• Support type: Regular Research Grants
• FAPESP's process: 20/02159-0 - Antiviral activity of AAK1 inhibitors against Oropouche infection
• Grantee: Karina Bispo dos Santos
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 19/06372-3 - Multiparametric flow cytometry
• Grantee: Alessandro dos Santos Farias
• Support type: Multi-user Equipment Program
• FAPESP's process: 17/26908-0 - The role of IRF-5 in Oropouche virus pathogenesis and neurovirulence
• Grantee: Pierina Lorencini Parise
• Support type: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 20/04579-7 - Study of the risk factors associated with greater severity to COVID-19 and mapping of the metabolic pathways required for the anti-SARS-CoV-2 response
• Grantee: Pedro Manoel Mendes de Moraes Vieira
• Support type: Regular Research Grants
• FAPESP's process: 20/04558-0 - Characterization of intrinsic risk factors and the development of new diagnostic and treatment alternatives for COVID-19
• Grantee: José Luiz Proença Módena
• Support type: Regular Research Grants
• FAPESP's process: 20/04583-4 - Impact of intestinal microbiota and its metabolites on SARS-CoV-2 infection
• Grantee: Marco Aurélio Ramirez Vinolo
• Support type: Regular Research Grants
• FAPESP's process: 19/14342-7 - Comparison of gamma variant tropism and pathogenicity versus original SARS CoV-2 in humanized K18-ACE2 mice: role of gut microbiota
• Grantee: Patrícia Brito Rodrigues
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/02448-2 - The role of the presence of IRF-5 in dendritic cells, macrophages and B lymphocytes to control infection by the Oropouche virus in murine model
• Grantee: Camila Lopes Simeoni
• Support type: Scholarships in Brazil - Scientific Initiation