Identification of novel mRNA isoforms associated with meat tenderness using RNA sequencing data in beef cattle

Author(s)

Magalhaes Muniz, Maria Malane ^{[1, 2]} ; Simielli Fonseca, Larissa Fernanda ^[1] ; dos Santos Silva, Danielly Beraldo ^[1] ; de Oliveira, Hinayah Rojas ^[2] ; Baldi, Fernando ^{[3, 1]} ; Chardulo, Artur Loyola ^{[3, 4]} ; Ferro, Jesus Aparecido ^{[3, 1]} ; Canovas, Angela ^[2] ; de Albuquerque, Lucia Galvao ^{[3, 1]}

Affiliation

^[1] Sao Paulo State Univ Unesp, Sch Agr & Vet Sci, Jaboticabal, SP - Brazil

^[2] Univ Guelph, Ctr Genet Improvement Livestock, Dept Anim Biosci, Guelph, ON - Canada

^[3] Natl Council Sci & Technol Dev CNPq, Brasilia, DF - Brazil

^[4] Sao Paulo State Univ Unesp, Coll Vet & Anim Sci, Botucatu, SP - Brazil

• Document type: Review article
• Source: MEAT SCIENCE; v. 173, MAR 2021.
• Web of Science Citations: 0

Abstract

The Warner-Bratzler shear force (WBSF) and myofibrillar fragmentation index (MFI) are complementary methodologies used to measure beef tenderness. Longissimus thoracis samples from the 20 most extreme bulls (out of 80 bulls set) for WBSF (tender (n = 10) and tough (n = 10)) and MFI (high (n = 10) and low (n = 10)) traits were collected to perform transcriptomic analysis using RNA-Sequencing. All analysis were performed through CLC Genomics Workbench. A total of 39 and 27 transcripts for WBSF and MFI phenotypes were DE, respectively. The possible DE novel mRNA isoforms, for WBSF and MFI traits, are myosin encoders (e.g. MYL1 and MYL6). In addition, we identified potential mRNA isoforms related to genes affecting the speed fibers degradation during the meat aging process. The DE novel transcripts are transcripted by genes with biological functions related to oxidative process, energy production and striated muscle contraction. The results suggest that the identified mRNA isoforms could be used as potential candidate to select animals in order to improve meat tenderness. (AU)

• FAPESP's process: 18/20026-8 - Multi-user equipment approved in grant 2017/10630-2: server
• Grantee: Lucia Galvão de Albuquerque
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 11/21241-0 - Study of the genetic variability of meat fatty acid profile in Nelore cattle finished in feedlot
• Grantee: Fernando Sebastián Baldi Rey
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 18/11154-2 - Identification of co-expression genes in Nellore cattle muscle tissue with divergent marbling and myofibrilar fragmentation index phenotypes, using RNA-Seq data
• Grantee: Maria Malane Magalhães Muniz
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 09/16118-5 - Genomic tools to genetic improvement of direct economic important traits in Nelore cattle
• Grantee: Lucia Galvão de Albuquerque
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/10630-2 - Genetic aspects of meat production quality, efficiency and sustainability in Nelore breed animals
• Grantee: Lucia Galvão de Albuquerque
• Support Opportunities: Research Projects - Thematic Grants