Lipid-matrix effects on tyrosinase immobilization in Langmuir and Langmuir-Blodgett films

Author(s)

MATHEUS S. PEREIRA ^[1] ; MATEUS D. MAXIMINO ^[2] ; CIBELY S. MARTIN ^[3] ; PEDRO H.B. AOKI ^[4] ; OSVALDO N. OLIVEIRA JR ^[5] ; PRISCILA ALESSIO ^[6]

Affiliation

^[1] Universidade Estadual Paulista/UNESP. Faculdade de Ciências e Tecnologia - Brasil

^[2] Universidade Estadual Paulista/UNESP. Faculdade de Ciências e Tecnologia - Brasil

^[3] Universidade Estadual Paulista/UNESP. Faculdade de Ciências e Tecnologia - Brasil

^[4] Universidade Estadual Paulista/UNESP. Faculdade de Ciências e Letras - Brasil

^[5] Universidade de São Paulo/USP. Instituto de Física de São Carlos - Brasil

^[6] Universidade Estadual Paulista/UNESP. Faculdade de Ciências e Tecnologia - Brasil

• Document type: Journal article
• Source: Anais da Academia Brasileira de Ciências; v. 93, n. 1 2021-03-26.

Abstract

Abstract The immobilization of the enzyme tyrosinase (Tyr) in lipid matrices can be explored to produce biosensors for detecting polyphenols, which is relevant for the food industry. Herein, we shall demonstrate the importance of the lipid composition to immobilize the enzyme tyrosinase in Langmuir-Blodgett (LB) films. Tyr could be incorporated into Langmuir monolayers of arachidic acid (AA), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (sodium salt) (DPPG), having as the main effect an expansion in the monolayers. Results from polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS) pointed to electrostatic interactions between the charged residues of Try and the lipid headgroups, in addition to changes in the order of lipid chains. The interaction between Tyr and DPPC in Langmuir monolayers can be correlated with the superior performance of DPPC/Tyr LB films used as biosensors to detect catechol by cyclic voltammetry. The molecular-level interactions assessed via PM-IRRAS are therefore believed to drive an immobilization process for Tyr in the lipid LB matrix and may serve as a general criterion to identify matrices that preserve enzyme activity. (AU)

• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/15019-0 - Combination of confocal fluorescence microscopy and surface-enhanced Raman scattering techniques: interaction, detection, and quantification of molecules of interest in biomimetic system.
• Grantee: Cibely da Silva Martin Sonvesso
• Support Opportunities: Scholarships in Brazil - Post-Doctoral