Association of Nd:YAG laser and calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion

Author(s)

Vinícius MAXIMIANO ^[1] ; Alana Cristina MACHADO ^[2] ; Raquel Marianna LOPES ^[3] ; Fernanda Ellen Mercatelli RABELO ^[4] ; Stephanie Assimakopoulos GARÓFALO ^[5] ; Denise Maria ZEZELL ^[6] ; Ana Cecilia Corrêa ARANHA ; Taís SCARAMUCCI

Affiliation

^[1] Universidade de São Paulo. Faculdade de Odontologia. Departamento de Dentística - Brasil

^[2] Universidade de São Paulo. Faculdade de Odontologia. Departamento de Dentística - Brasil

^[3] Universidade de São Paulo. Faculdade de Odontologia. Departamento de Dentística - Brasil

^[4] Universidade de São Paulo. Faculdade de Odontologia. Departamento de Dentística - Brasil

^[5] Universidade de São Paulo. Faculdade de Odontologia. Departamento de Dentística - Brasil

^[6] Universidade de São Paulo. Instituto de Pesquisas Energéticas e Nucleares - Brasil

• Document type: Journal article
• Source: Journal of Applied Oral Science; v. 29, 2021-03-31.

Abstract

Abstract Objective To evaluate the efficacy of Nd:YAG laser associated with calcium-phosphate desensitizing pastes on dentin permeability and tubule occlusion after erosive/abrasive challenges. Methodology Dentin specimens were exposed to 17% ethylene diamine tetra-acetic acid (EDTA) solution for 5 min and randomly allocated into five groups: G1, control (no treatment); G2, Nd:YAG laser (1 W, 10 Hz, 100 mJ, 85 J/cm2); G3, Laser + TeethmateTM Desensitizer; G4, Laser + Desensibilize Nano P; and G5, Laser+Nupro®. Specimens underwent a 5-day erosion-abrasion cycling. Hydraulic conductance was measured post-EDTA, post-treatment, and post-cycling. Post-treatment and post-cycling permeability (%Lp) was calculated based on post-EDTA measurements, considered 100%. Open dentin tubules (ODT) were calculated at the abovementioned experimental moments using scanning electron microscopy and ImageJ software (n=10). Data were analyzed using two-way repeated measures ANOVA and Tukey’s test (α=0.05). Results G1 presented the highest %Lp post-treatment of all groups (p<0.05), without significantly differences among them. At post-cycling, %Lp significantly decreased in G1, showed no significant differences from post-treatment in G3 and G4, and increased in G2 and G5, without significant differences from G1 (p>0.05). We found no significant differences in ODT among groups (p>0.05) post-EDTA. At post-treatment, treated groups did not differ from each other, but presented lower ODT than G1 (p<0.001). As for post-cycling, we verified no differences among groups (p>0.05), although ODT was significantly lower for all groups when compared to post-EDTA values (p<0.001). Conclusion All treatments effectively reduced dentin permeability and promoted tubule occlusion after application. Combining Nd YAG laser with calcium-phosphate pastes did not improve the laser effect. After erosive-abrasive challenges, treatments presented no differences when compared to the control. (AU)

• FAPESP's process: 17/50332-0 - Scientific, technological and infrastructure qualification in radiopharmaceuticals, radiation and entrepreneurship for health purposes (PDIp)
• Grantee: Marcelo Linardi
• Support Opportunities: Research Grants - State Research Institutes Modernization Program
• FAPESP's process: 17/02341-0 - Evaluation of the effectiveness of in-office desensitizing agents in the hydraulic condutance of dentin
• Grantee: Fernanda Ellen Mercatelli Rebelo
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/24714-3 - Effect of the order of application of dentifrices and solutions containing fluorides and tin in the progression of erosive wear on enamel and bovine dentin - in situ study
• Grantee: Alana Cristina Machado
• Support Opportunities: Scholarships in Brazil - Doctorate