Due to changes in lifestyle in the modern world and the world's population becoming older, an increase in the occurrence of non-carious cervical lesions is increasing. As a result, dentin hypersensitivity can be highlighted as a common complaint among adults and represents one of the most critical and persistent problems in dentistry. This study has two distinct parts. The first part, longitudinal randomized, clinical and in vivo aims to evaluate different protocols of treatment for dentin hypersensitivity with low power laser (with different strengths), high-power laser, desensitizing agent and associations, for a period of 18 months. The lesions were divided into nine groups (n = 10), totalizing 90 treat and evaluated teeth: G1: Gluma Desensitizer (Heraeus Kulzer), G2: Low Level Laser with low dosage (Photon Lase, DMC, irradiations on three cervical vestibular points and an apical point: 30mW, 10J/cm2, 9 seconds per point, with the wavelength of 810nm, in three sessions with 72 hours intervals), G3: Low Level Laser with high dosage (one cervical point and an apical point application: 100 mW, 90 J/cm2, 11 seconds by point, with the wavelength of 810nm. Three sessions were performed with 72 hours interval), G4: Low Level Laser with low dosage + Gluma Desensitizer, G5: Low Level Laser with high dosage + Gluma Desensitizer, G6: Nd:YAG Laser (Power LaserTM ST6, Lares Research®, in contact made with the protocol of 1.0 W, 10 Hz and 100 mJ, H 85 J/cm2, with the wavelength of 1064nm. Four irradiations were performed, each for 15 seconds, in mesio-distal and ocluso-apical directions, totaling an irradiation of 60 seconds with 10 seconds intervals), G7: Nd:YAG Laser + Gluma Desensitizer, G8: Nd:YAG Laser + Low Level Laser with low dosage, G9: Nd:YAG Laser + Low Level Laser with high dosage. The sensitivity level of each volunteer will be evaluated by visual analog scale (VAS) with the aid of cold air stimuli and exploration probe after 12 and 18 months of treatment. The data will be collected and submitted to statistical analysis. After the execution of clinical work, the in vitro part will be held on not erupted human third molars, which will receive three different treatments: Nd: YAG Laser, Desensitizing Gluma Desensitizer and their associations. After the samples preparation, those will receive different cycles brushing and will be analyzed in scanning Electronic Microscopy and in a Perfilometer. So it will be possible to do quantitative and qualitative analyses of the samples.
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