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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The role of chronic muscle (in)activity on carnosine homeostasis: a study with spinal cord-injured athletes

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Author(s):
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Nemezio, Kleiner [1] ; Yamaguchi, Guilherme de Carvalho [1] ; Boito Ramkrapes, Ana Paula [2] ; Schulz, Mariane Leichsenring [3] ; Baptista, Igor Luchini [4] ; Riani, Luiz Augusto [1] ; Goncalves, Livia de Souza [1] ; Sale, Craig [5] ; Gennari de Medeiros, Marisa Helena [3] ; Gualano, Bruno [1] ; Artioli, Guilherme Giannini [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Fac Med, Sch Phys Educ & Sport, Div Reumatol, Appl Physiol & Nutr Res Grp, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Fac Phys Educ, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
[4] Univ Estadual Campinas, Fac Ciencias Aplicadas, Sao Paulo - Brazil
[5] Nottingham Trent Univ, Sport Hlth & Performance Enhancement Res Ctr, Musculoskeletal Physiol Res Grp, Nottingham - England
Total Affiliations: 5
Document type: Journal article
Source: AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY; v. 320, n. 6, p. R824-R832, JUN 2021.
Web of Science Citations: 0
Abstract

To examine the role of chronic (in)activity on muscle carnosine (MCarn) and how chronic (in)activity affects MCarn responses to 13-alanine supplementation in spinal cord-injured athletes, 16 male athletes with paraplegia were randomized (2:1 ratio) to receive 13-alanine (n = 11) or placebo (PL, n = 5). They consumed 6.4 g/day of 13-alanine or PL for 28 days. Muscle biopsies of the active deltoid and the inactive vastus lateralis (VL) were taken before and after supplementation. MCarn in the VL was also compared with the VL of a group of individuals without paraplegia (n = 15). MCarn was quantified in whole muscle and in pools of individual fibers by high-performance liquid chromatography. MCarn was higher in chronically inactive VL vs. well-trained deltoid (32.0 +/- 12.0 vs. 20.5 +/- 6.1 mmol/kg DM; P = 0.018). MCarn was higher in inactive vs. active VL (32.0 +/- 12.0 vs. 21.2 +/- 7.5 mmol/kg DM; P = 0.011). In type-I fibers, MCarn was significantly higher in the inactive VL than in the active deltoid (38.3 +/- 4.7 vs. 27.3 +/- 11.8 mmol/kg DM, P = 0.014). MCarn increased similarly between inactive VL and active deltoid in the 13-alanine group (VL: 68.9 +/- 55.1%, P = 0.0002; deltoid: 90.5 +/- 51.4%, P < 0.0001), with no changes in the PL group. MCarn content was higher in the inactive VL than in the active deltoid and the active VL, but this is probably a consequence of fiber type shift (type I to type II) that occurs with chronic inactivity. Chronically inactive muscle showed an increase in MCarn after BA supplementation equally to the active muscle, suggesting that carnosine accretion following 13-alanine supplementation is not influenced by muscle inactivity. (AU)

FAPESP's process: 14/11948-8 - Life without carnosine: development and characterization of a KO rat model for studying the physiological role of carnosine and its implications to physical exercise and muscle metabolism
Grantee:Guilherme Giannini Artioli
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 19/25032-9 - The role of carnosine on Ca2+ handling, control of oxidative stress and protection against protein glycation: advances and applications of the study life without carnosine
Grantee:Hamilton Augusto Roschel da Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 17/13552-2 - Reducing sedentary time in clinical populations: the take a stand for health study
Grantee:Bruno Gualano
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 15/23176-2 - The kinetics of muscle carnosine washout after beta-alanine supplementation
Grantee:Guilherme de Carvalho Yamaguchi
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 13/14746-4 - Carnosine metabolism in skeletal muscle: a multi-approach study
Grantee:Bruno Gualano
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/12236-5 - Effects of caffeine exposure on mitophagic pathways: actions over myogenic progenitor cells differentiation
Grantee:Igor Luchini Baptista
Support Opportunities: Regular Research Grants