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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Zein based-nanoparticles loaded botanical pesticides in pest control: An enzyme stimuli-responsive approach aiming sustainable agriculture

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Author(s):
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Monteiro, Renata Aparecida [1] ; Camara, Marcela Candido [1] ; de Oliveira, Jhones Luiz [2] ; Ramos Campos, Estefania Vangelie [3] ; Carvalho, Lucas Braganca [1] ; de Freitas Proenca, Patricia Luiza [1] ; Guilger-Casagrande, Mariana [4] ; Lima, Renata [4] ; do Nascimento, Joacir [2] ; Goncalves, Kelly Cristina [2] ; Polanczyk, Ricardo Antonio [2] ; Fraceto, Leonardo Fernandes [4]
Total Authors: 12
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, BR-18087180 Sorocaba, SP - Brazil
[2] Sao Paulo State Univ UNESP, Fac Agron & Vet Sci, BR-14884900 Jaboticabal, SP - Brazil
[3] Fed Univ ABC UFABC, Human & Nat Sci Ctr, BR-09210580 Santo Andre, SP - Brazil
[4] Univ Sorocaba UNISO, Lab Bioact Assessment & Toxicol Nanomat LABiToN, BR-18023000 Sorocaba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF HAZARDOUS MATERIALS; v. 417, SEP 5 2021.
Web of Science Citations: 2
Abstract

Nanoencapsulation of biopesticides is an important strategy to increase the efficiency of these compounds, reducing losses and adverse effects on non-target organisms. This study describes the preparation and characterisation of zein nanoparticles containing the botanical compounds limonene and carvacrol, responsive to proteolytic enzymes present in the insects guts. The spherical nanoparticles, prepared by the anti-solvent precipitation method, presented in the nanoparticle tracking analysis (NTA) a concentration of 4.7 x 1012 +/- 1.3 x 1011 particles.mL-1 and an average size of 125 +/- 2 nm. The formulations showed stability over time, in addition to not being phytotoxic to Phaseolus vulgaris plants. In vivo tests demonstrated that formulations of zein nanoparticles containing botanical compounds showed higher mortality to Spodoptera frugiperda larvae. In addition, the FTIC probe (fluorescein isothiocyanate) showed wide distribution in the larvae midgut, as well as being identified in the feces. The trypsin enzyme, as well as the enzymatic extract from insects midgut, was effective in the degradation of nanoparticles containing the mixture of botanical compounds, significantly reducing the concentration of nanoparticles and the changes in size distribution. The zein degradation was confirmed by the disappearance of the protein band in the electrophoresis gel, by the formation of the lower molecular weight fragments and also by the greater release of FTIC after enzymes incubation. In this context, the synthesis of responsive nanoparticles has great potential for application in pest management, increasing the selectivity and specificity of the system and contributing to a more sustainable agriculture. (AU)

FAPESP's process: 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity
Grantee:Leonardo Fernandes Fraceto
Support type: Research Projects - Thematic Grants
FAPESP's process: 18/23608-8 - Development of labelled nanopesticides aiming studies of mechanism of action and toxicity
Grantee:Lucas Bragança de Carvalho
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/21142-1 - Encapsulation of Bacillus thuringiensis (Bacillales: Bacillaceae) in polymeric microparticles and association with nanoencapsulated chemical agent: evaluation of biological activity and mechanisms of action
Grantee:Jhones Luiz de Oliveira
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/13328-5 - Development and evaluation of biogenic metal nanoparticles (silver, titanium and iron) based on Trichoderma harzianum for agricultural application
Grantee:Renata de Lima
Support type: Regular Research Grants
FAPESP's process: 20/05816-2 - Study of the capping of biogenic nanoparticles and their correlation with toxicity and activity in the control of phytopathogens
Grantee:Mariana Guilger Casagrande
Support type: Scholarships in Brazil - Post-Doctorate