Evaluation of residual antimicrobial activity and acute toxicity during the degradation of gatifloxacin by ozonation

Author(s)

Caianelo, Marlon ^[1] ; Rodrigues-Silva, Caio ^[2] ; Maniero, Milena Guedes ^[1] ; Diniz, Vinicius ^[2] ; Spina, Mylena ^[1] ; Guimaraes, Jose Roberto ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Civil Engn Architecture & Urban Design, POB 6143, BR-13083889 Campinas - Brazil

^[2] Univ Estadual Campinas, Dept Analyt Chem, Inst Chem, POB 6154, BR-13084971 Campinas, SP - Brazil

• Document type: Journal article
• Source: WATER SCIENCE AND TECHNOLOGY; v. 84, n. 1, p. 225-236, JUL 1 2021.
• Web of Science Citations: 0

Abstract

The concerns regarding the occurrence of pharmaceuticals in wastewater treatment plants have been increased in the last decades. Gatifloxacin (GAT), the fourth generation of fluoroquinolones, has been widely used to treat both Gram-positive and Gram-negative bacteria and has a limited metabolization. The present study aimed to evaluate the ozonation as a technique to degrade GAT. A UHPLC-MS/MS method was used to quantify the residual of GAT and to assess its degradation products. The removal efficiency was higher at alkaline condition (pH = 10), reaching up to 99% of GAT after 4 min. It was also observed that the first ozone attack on the GAT molecule was through the carboxylic group. In contrast, at acid conditions (pH = 3), the ozone attack was first to the piperazinyl ring. The antimicrobial activity was evaluated using Escherichia coli and Bacillus subtilis as test organisms, and it was observed that the residual activity reduced most at the alkaline conditions. In contrast, the best condition to remove the residual toxicity evaluated for the marine bacteria V. fischeri was the acidic one. Due to this, the ozonation seemed to be an exciting process to remove GAT at aqueous media. (AU)

• FAPESP's process: 18/03571-2 - Pharmaceuticals in the Aquatic Environment.
• Grantee: Susanne Rath
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/50951-4 - INCT 2014: Advanced Analytical Technologies
• Grantee: Celio Pasquini
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/09543-7 - Residues of veterinary drugs in the environment
• Grantee: Susanne Rath
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/16622-3 - Identification of intermediates formed during degradation of veterinary drugs by advanced oxidation processes
• Grantee: Caio Alexandre Augusto Rodrigues da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral