In vitro and in vivo osteogenic potential of niobium-doped 45S5 bioactive glass: A comparative study

Author(s)

Lopes, Joao H. ^[1] ; Souza, Lucas P. ^[2] ; Domingues, Juliana A. ^[2] ; Ferreira, V, Filipe ; Hausen, Moema de Alencar ^[3] ; Camilli, Jose A. ^[2] ; Martin, Richard A. ^{[4, 5]} ; de Rezende Duek, Eliana A. ^[3] ; Mazali, Italo O. ^[6] ; Bertran, Celso A. ^[7]

Affiliation

^[1] Aeronaut Inst Technol ITA, Dept Chem, Div Fundamental Sci IEF, BR-12228900 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, UNICAMP, BR-13083862 Campinas, SP - Brazil

^[3] Pontifical Catholic Univ Sao Paulo, Dept Physiol Sci, Biomat Lab, Sorocaba - Brazil

^[4] Aston Univ, Sch Engn, Birmingham, W Midlands - England

^[5] Aston Univ, Aston Res Ctr Hlth Ageing, Birmingham, W Midlands - England

^[6] Univ Estadual Campinas, Dept Inorgan Chem, Inst Chem, UNICAMP, Campinas, SP - Brazil

^[7] Univ Estadual Campinas, Dept Phys Chem, Inst Chem, UNICAMP, Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 108, n. 4, p. 1372-1387, MAY 2020.
• Web of Science Citations: 5

Abstract

In vitro and in vivo experiments were undertaken to evaluate the solubility, apatite-forming ability, cytocompatibility, osteostimulation, and osteoinduction for a series of Nb-containing bioactive glass (BGNb) derived from composition of 45S5 Bioglass. Inductively coupled plasma optical emission spectrometry (ICP-OES) revealed that the rate at which Na, Ca, Si, P, and Nb species are leached from the glass decrease with the increasing concentration of the niobium oxide. The formation of apatite as a function of time in simulated body fluid was monitored by 31P Magic Angle Spinning (MAS) Nuclear magnetic resonance spectroscopy. Results showed that the bioactive glasses: Bioglass 45S5 (BG45S5) and 1 mol%-Nb-containing-bioactive glass (BGSN1) were able to grow apatite layer on their surfaces within 3 h, while glasses with higher concentrations of Nb2O5 (2.5 and 5 mol%) took at least 12 h. Nb-substituted glasses were shown to be compatible with bone marrow-derived mesenchymal stem cells (BMMSCs). Moreover, the bioactive glass with 1 mol% Nb2O5 significantly enhanced cell proliferation after 4 days of treatment. Concentrations of 1 and 2.5 mol% Nb2O5 stimulated osteogenic differentiation of BMMSCs after 21 days of treatment. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their osteoconductivity and osteostimulation. Two morphometric parameters were analyzed: (a) thickness of new-formed bone layer and (b) area of new-formed subperiostal bone. Results showed that BGNb bioactive glass is osteoconductive and osteostimulative. Therefore, these results indicate that Nb-substituted glass is suitable for biomedical applications. (AU)

• FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
• Grantee: Anderson de Oliveira Lobo
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 11/09240-9 - Calcium phosphate cement otimization for application in orthopedics and tissue engineering
• Grantee: Mariana Motisuke
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/05394-9 - Bioglasses derived from 45S5: effects of Nb2O5 or surface modification with Ca2+ on the glass structure and bioactivity
• Grantee: João Henrique Lopes
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/00863-0 - Processing and characterization of B-TCP/Al2O3 nanostructured scaffolds for biomedical applications
• Grantee: Eliandra de Sousa Trichês
• Support Opportunities: Regular Research Grants