ntiplatelet Activity of Cucurbita maxim

Author(s)

Sanzana, Sigrid ^[1] ; Rodriguez, Lyanne ^[2] ; Barraza Barrionuevo, Hayleen ^[3] ; Albornoz Poblete, Cesar ^[3] ; Marostica Junior, Mario Roberto ^{[4, 5, 6]} ; Fuentes, Eduardo ^[2] ; Palomo, Ivan ^[2]

Affiliation

^[1] Univ Antofagasta, Dept Med Technol, Antofagasta - Chile

^[2] Univ Talca, Fac Hlth Sci, Dept Clin Biochem & Immunohematol, Thrombosis Res Ctr, Med Technol Sch, Talca 3460000 - Chile

^[3] Univ Antofagasta, Dept Food & Nutr Sci, Fac Hlth Sci, Antofagasta - Chile

^[4] Univ Estadual Campinas, Sch Food Engn, Campinas, SP - Brazil

^[5] Ibero Amer Network Integrated Use Underutilized I, Cambridge - England

^[6] Univ Campinas UNICAMP, Fac Sch Engn FEA, Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF MEDICINAL FOOD; AUG 2021.
• Web of Science Citations: 1

Abstract

Natural extracts constitute an important source in the prevention of noncommunicable diseases, such as cardiovascular diseases. The pumpkin, Cucurbita maxima, is widely consumed in Chile. Pumpkin seeds, despite having crude protein, lipids, and carbohydrates, are regarded as agro-industrial waste. In this work, we correlated the antiplatelet activity of aqueous, ethanolic, and methanolic extracts from pumpkin seeds with their bioactive compounds. In vitro platelet aggregation and activation studies were performed by turbidimetry and flow cytometry, respectively. Results reveal that the extracts inhibited, in a dose-dependent manner, platelet aggregation induced by adenosine diphosphate, thrombin receptor activator peptide 6 (TRAP-6), and collagen. Pumpkin seed extracts inhibited P-selectin secretion and glycoprotein IIb/IIIa activation on TRAP-6-activated platelets. They were found to be rich in fatty acids and a powerful source of plant-based protein, which could be related to the high antiplatelet potential identified in extracts. This research demonstrated that pumpkin seed extracts could be a candidate in the prevention of thrombotic events. (AU)

• FAPESP's process: 18/11069-5 - Action of Brazilian berries on metabolic parameters and cognitive performance of obese animals
• Grantee: Mário Roberto Maróstica Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/50333-1 - COBRA: a novel compound discovery pipeline for rapid and cost-effective identification of bioactives with health beneficial effects in Brazilian crops
• Grantee: Glaucia Maria Pastore
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/13320-9 - Effect of pulp oil and tea of leaves from avocado Hass variety associated with physical activity in the treatment of obesity induced by high-fat diet
• Grantee: Mário Roberto Maróstica Junior
• Support Opportunities: Regular Research Grants