Low Temperature Effect on the Endocrine and Circadian Systems of Adult Danio rerio

Author(s)

Sua-Cespedes, Cristhian D. ^[1] ; David, Daniela Dantas ^[1] ; Souto-Neto, Jose A. ^[1] ; Lima, Otoniel Goncalves ^[1] ; Moraes, Maria Nathalia ^[2] ; de Assis, Leonardo V. Monteiro ^{[1, 3]} ; Castrucci, Ana Maria de Lauro ^{[4, 1]}

Affiliation

^[1] Univ Sao Paulo, Inst Biosci, Dept Physiol, Lab Comparat Physiol Pigmentat, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Lab Neurobiol, Sao Paulo - Brazil

^[3] Lubeck Univ, Inst Neurobiol, Ctr Brain Behav & Metab, Lubeck - Germany

^[4] Univ Virginia, Dept Biol, Charlottesville, VA 22904 - USA

• Document type: Journal article
• Source: FRONTIERS IN PHYSIOLOGY; v. 12, NOV 24 2021.
• Web of Science Citations: 0

Abstract

The control of the biological rhythms begins with the activation of photo- and thermosensitive cells located in various organs of the fish such as brain, eye, and skin, but a central clock is still to be identified in teleosts. Thermal changes are stressors which increase cortisol and affect the rhythm of other hormones such as melatonin and growth hormone (GH), in both endo- and ectothermic organisms. Our aim was to investigate how temperature (23 degrees C for 6 days) lower than the optimal (28 degrees C) modulates expression of several gene pathways including growth hormone (gh1) and its receptors (ghra, ghrb), insulin-like growth factor1 (igf1a, igf1b) and its receptors (igf1ra, igf1rb), cortisol and its receptor (gr), the limiting enzyme of melatonin synthesis (arylalkylamine N-acetyltransferase, aanat) and melatonin receptors (mtnr1aa, mtnr1bb), as well as their relationship with clock genes in Danio rerio in early light and early dark phases of the day. Lower temperature reduced the expression of the hormone gene gh1, and of the related receptors ghra, ghrb, igf1ra, and igf1rb. Cortisol levels were higher at the lower temperature, with a decrease of its receptor (gr) transcripts in the liver. Interestingly, we found higher levels of aanat transcripts in the brain at 23 degrees C. Overall, lower temperature downregulated the transcription of hormone related genes and clock genes. The results suggest a strong correlation of temperature challenge with the clock molecular mechanism and the endocrine systems analyzed, especially the growth hormone and melatonin axes, in D. rerio tissues. (AU)

• FAPESP's process: 18/23043-0 - Photo- and termo-reception systems in the Blue Crab, Callinectes sapidus (Crustacea Brachyura), and their relationship with the molt cycle
• Grantee: Daniela Dantas David
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/16511-8 - Involvement of the photosensitive and temporal controlling systems in the development, progression, and metastasis of malignant melanoma: an investigation of novel therapeutic targets
• Grantee: Leonardo Vinícius Monteiro de Assis
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/26651-9 - Glaucoma as a model of temporal information disruption: impact on the metabolism
• Grantee: Maria Nathália de Carvalho Magalhães Moraes Figueira Borges
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 18/14728-0 - Melanopsin as the UVA photoreceptor and its relationship with pigmentation, DNA repair, biological clock and components of the HPA axis: a novel pharmacological target?
• Grantee: Ana Maria de Lauro Castrucci
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/24615-5 - Breaking a paradigm? Melanopsin, a canonical photo-pigment, acting as sensor to entrain the clock in light unexposed organs, and its putative interaction with TRP channels: a trans-disciplinary study involving physiological and pathological aspects
• Grantee: Ana Maria de Lauro Castrucci
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/23915-8 - Cold challenge and its effects on canonical thermosensation processes in BAT and their possible interaction with TRP channels
• Grantee: Otoniel Gonçalves de Lima
• Support Opportunities: Scholarships in Brazil - Master