| Full text | |
| Author(s): Show less - |
Bondancia, Thalita J.
;
Soares, Andrey Coatrini
;
Popolin-Neto, Mario
;
Gomes, Nathalia O.
;
Raymundo-Pereira, Paulo A.
;
Barud, Hernane S.
;
Machado, Sergio A. S.
;
Ribeiro, Sidney J. L.
;
Melendez, Matias E.
;
Carvalho, Andre L.
;
Reis, Rui M.
;
Paulovich, Fernando, V
;
Oliveira Jr, Osvaldo N.
Total Authors: 13
|
| Document type: | Journal article |
| Source: | BIOMATERIALS ADVANCES; v. 134, p. 7-pg., 2022-03-01. |
| Abstract | |
Low-cost sensors to detect cancer biomarkers with high sensitivity and selectivity are essential for early diagnosis. Herein, an immunosensor was developed to detect the cancer biomarker p53 antigen in MCF7 lysates using electrical impedance spectroscopy. Interdigitated electrodes were screen printed on bacterial nanocellulose substrates, then coated with a matrix of layer-by-layer films of chitosan and chondroitin sulfate onto which a layer of anti-p53 antibodies was adsorbed. The immunosensing performance was optimized with a 3-bilayer matrix, with detection of p53 in MCF7 cell lysates at concentrations between 0.01 and 1000 U-cell. mL(-1), and detection limit of 0.16 U-cell mL(-1). The effective buildup of the immunosensor on bacterial nanocellulose was confirmed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and surface energy analysis. In spite of the high sensitivity, full selectivity with distinction of the p53-containing cell lysates and possible interferents required treating the data with a supervised machine learning approach based on decision trees. 'this allowed the creation of a multidimensional calibration space with 11 dimensions (frequencies used to generate decision tree rules), with which the classification of the p53-containing samples can be explained. (AU) | |
| FAPESP's process: | 20/09587-8 - Development of electrochemical devices based on carbon and silver nanostructures to detect endocrine disruptors in quantities of water supply |
| Grantee: | Nathalia Oezau Gomes |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 19/01777-5 - Manufacture of disposable electrochemical devices composed of hollow carbon nanospheres and carbon nanowires decorated with silver nanoparticles for the quantification of endocrine disruptors in supply water samples |
| Grantee: | Sergio Antonio Spinola Machado |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/18953-8 - Nanostructured films applied in microfluidic biosensors to mastitis detection |
| Grantee: | Andrey Coatrini Soares |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/01919-6 - Design and fabrication of nanostructured flexible devices for biomarkers detection |
| Grantee: | Paulo Augusto Raymundo Pereira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |