| Full text | |
| Author(s): |
Rodrigues, Raul Torres
;
Caseli, Luciano
Total Authors: 2
|
| Document type: | Journal article |
| Source: | Thin Solid Films; v. 753, p. 5-pg., 2022-07-01. |
| Abstract | |
Pectinase, an enzyme with poor surface activity, was adsorbed from a saline aqueous subphase at the water interface through the salting-out effect. Tensiometry measurements confirmed the formation of stable films at the interface, and surface potential-area isotherms confirmed the orientation of the enzyme dipoles at the interface. Polarization modulation reflection-absorption infrared spectroscopy provided information on the secondary structure of the enzyme, which showed negligible loss of its native conformation, with a majority presence of beta-sheets. We did not observe relevant formation of aggregates by means of cycles of compression-expansion as well as through Brewster Angle Microscopy. The floating enzyme monolayer could be transferred to solid supports as revealed with nanogravimmetry and fluorescence spectroscopy. (AU) | |
| FAPESP's process: | 18/22091-1 - Langmuir and Langmuir-Blodgett films of lipid containing pectinase for pectin sensors |
| Grantee: | Raul Torres Rodrigues |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 14/50869-6 - INCT 2014: on Organic Electronics |
| Grantee: | Roberto Mendonça Faria |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/03239-0 - Nanostructured interfaces for the investigation of bioactive substances in cell membrane models and for the construction of optoelectronic devices |
| Grantee: | Luciano Caseli |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |