Brazilian Berry Extract Differentially Induces Inflammatory and Immune Responses in Androgen Dependent and Independent Prostate Cancer Cells

Author(s)

Kido, Larissa Akemi ; Rossetto, Isabela Maria Urra ; Baseggio, Andressa Mara ; Chiarotto, Gabriela Bortolanza ; Alves, Leticia Ferreira ; Santos, Felipe Rabelo ; Lamas, Celina de Almeida ; Marostica, Mario Roberto ; Cagnon, Valeria Helena Alves

• Document type: Journal article
• Source: JOURNAL OF CANCER PREVENTION; v. 27, n. 3, p. 10-pg., 2022-09-30.

Abstract

Jaboticaba is a Brazilian berry, which is rich in fibers and bioactive compounds and shows high antioxidant and antiproliferative ac-tivities. Prostate cancer (PCa) is the second most common type of cancer among men and its progression is influenced by andro-gens and inflammation. Previous studies reported the ability of the jaboticaba to modulate pathways involved in prostate diseases. The main objective of this study was to provide significant data about molecular targets of the jaboticaba peel extract (JPE) and its mechanisms of action in PCa cell lines with different androgenic status (LNCaP and PC-3). The results showed that JPE was able to decrease cell viability in both cell lines. LNCaP showed more sensitivity to JPE exposure, indicating the efficacy of the JPE treat-ment in terms of androgen responsiveness. JPE showed a distinct hormone dependent effect on the NF -KB signaling, with reduced NF -KB levels for LNCaP and increased NF -KB levels in PC-3 cells. Mechanisms related to cell death by apoptosis were stimulated after the JPE treatment, modulating B-cell lymphoma 2 and BAX for LNCaP and PC-3. Particularly for PC-3, the JPE treatment re-sulted in cytokine-cytokine receptor interaction activation mostly by up regulating pro-inflammatory, pro-angiogenic, immunostimu-latory and immunosuppressive genes. Also, a set of genes related to angiogenesis and metastasis were down-regulated by JPE. In conclusion, JPE exerted an antitumor effect on PCa for both cell lines which can be enhanced if androgenic reliance is considered. (AU)

• FAPESP's process: 18/04579-7 - Jaboticaba Extract: In vivo response of prostatic adenocarcinoma in TRAMP mice associated with hyperlipic diet intake and in vitro response of tumoral human prostatic cells
• Grantee: Valéria Helena Alves Cagnon Quitete
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/50333-1 - COBRA: a novel compound discovery pipeline for rapid and cost-effective identification of bioactives with health beneficial effects in Brazilian crops
• Grantee: Glaucia Maria Pastore
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/13465-8 - Lund University and State University of Campinas partnership for studies on cardiometabolic disease-preventive functional foods and diets
• Grantee: Mário Roberto Maróstica Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/13320-9 - Effect of pulp oil and tea of leaves from avocado Hass variety associated with physical activity in the treatment of obesity induced by high-fat diet
• Grantee: Mário Roberto Maróstica Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/11069-5 - Action of Brazilian berries on metabolic parameters and cognitive performance of obese animals
• Grantee: Mário Roberto Maróstica Junior
• Support Opportunities: Regular Research Grants