Optimization of a Microwave-Assisted Extraction Method for the Recovery of the Anthocyanins from Jabuticaba By-Products

Author(s)

Barroso, Tiago L. C. T. ; Castro, Luiz E. N. ; F. Barbero, Gerardo ; Palma, Miguel ; Carrera, Ceferino ; Rostagno, Mauricio A. ; Forster-Carneiro, Tania

• Document type: Journal article
• Source: AGRONOMY-BASEL; v. 13, n. 2, p. 13-pg., 2023-02-01.

Abstract

In this study, a Box-Behnken Design (BBD) has been used to optimize the recovery of bioactive compounds from jabuticaba (Myrciaria cauliflora) by-products through microwave-assisted extraction (MAE). Thus, the Box-Behnken (BBD) experimental design was followed by a response surface methodology (RSM) that would allow investigating the influence of the four independent variables (temperature, solvent composition, pH, and sample-solvent ratio) that have been considered. The results revealed that temperature and solvent composition (%MeOH) were the most significant factors regarding the yields of anthocyanins obtained from the studied plant matrix. The established optimized conditions and 10-min-extraction time resulted in yields of 9.70 +/- 0.28 mg g(-1) of total anthocyanins. The method exhibited good repeatability and intermediate precision, with RSD variations lower than 5% for both parameters. The developed process was also able to extract and identify anthocyanins in commercial samples (jabuticaba pulp and jam). The results obtained from the optimized MAE method suggest that this technique is not only efficient for the recovery of anthocyanins from jabuticaba by-products, but it is also beneficial for a circular economy approach. (AU)

• FAPESP's process: 22/02039-0 - Recovery biocomponents of jabuticaba peel through microwave-assisted solid phase extraction (MASPE) and developing a rapid method of analysis in ultra-high performance liquid chromatography
• Grantee: Tiago Linhares Cruz Tabosa Barroso
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 18/14938-4 - Waste2energy: integration of supercritical technologies and anaerobic digestion through an automated system for the production of biofuel precursors
• Grantee: Tânia Forster Carneiro
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 20/16248-5 - Purification of jabuticaba waste extracts by adsorbents and biochar using supercritical technology
• Grantee: Tiago Linhares Cruz Tabosa Barroso
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 21/04096-9 - Integration of the extraction/hydrolysis processes in subcritical water and purification for the valuation of grape bagasse in a biorrefinery concept
• Grantee: Luiz Eduardo Nochi de Castro
• Support Opportunities: Scholarships in Brazil - Doctorate