Effect of denture liners surface modification with Equisetum giganteum and Punica granatum on Candida albicans biofilm inhibition

Author(s)

Garcia, Cindy Ruiz ; Ueda, Thais Yumi ; da Silva, Rafaela Alves ; Cano, Isadora Prado ; Saldanha, Luiz Leonardo ; Dokkedal, Anne Ligia ; Porto, Vinicius Carvalho ; Urban, Vanessa Migliorini ; Neppelenbroek, Karin Hermana

• Document type: Journal article
• Source: THERAPEUTIC DELIVERY; v. 13, n. 3, p. 10-pg., 2022-02-23.

Abstract

Aim: This study investigated the effect of denture liners surface modification with Equisetum giganteum (EG) and Punica granatum (PG) on Candida albicans biofilm inhibition supposing its usage as a sustained-release therapeutical delivery system for Candida-associated denture stomatitis. Materials & methods: C. albicans biofilm (SC5314 or ATCC 90028) was formed on soft liners superficially modified by a primer mixed to drugs at minimum inhibitory concentrations (0.100 g for EG and PG or 0.016 g for nystatin per ml of primer). After 24 h, 7 or 14 days, antibiofilm activity was evaluated by colony-forming unit counts. Results: Not all groups were equi-efficient to nystatin after 24 h and 7 days. After 14 days, EG and PG efficacies were not different from nystatin (almost 100% inhibition). Conclusion: The proposed protocol presents a promising option to allopathic drugs for Candida-associated denture stomatitis treatment. (AU)

• FAPESP's process: 17/07314-1 - Clinical evaluation of antifungal addition to temporary soft liner in the treatment of denture stomatitis
• Grantee: Karin Hermana Neppelenbroek
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/08702-2 - Effect of phytotherapeutic drugs incorporated into temporary denture soft lining materials on Candida albicans biofilm
• Grantee: Thaís Yumi Ueda
• Support Opportunities: Scholarships in Brazil - Scientific Initiation