Residue Depletion Profile and Estimation of Withdrawal Period for Sulfadimethoxine and Ormetoprim in Edible Tissues of Nile Tilapia (Oreochromis sp.) on Medicated Feed

Author(s):Show less -

de Freitas, Lucas Victor Pereira ; da Mota, Carlos Augusto Alvarenga ; Damaceno, Marina Alves ; Silveira, Juliana Grell Fernandes ; Portela, Ana Carolina Vellosa ; Campanharo, Sarah Chagas ; da Silva, Agnaldo Fernando Baldo ; Assane, Inacio Mateus ; Pilarski, Fabiana ; Sasanya, James Jacob ; Paschoal, Jonas Augusto Rizzato

• Document type: Journal article
• Source: ANIMALS; v. 13, n. 15, p. 13-pg., 2023-08-01.

Abstract

Simple Summary The use of veterinary drugs in fish farms is required to maintain animal health, minimize economic losses, and increase productivity. This study investigated the residue depletion profile of sulfadimethoxine (SDM) and ormetoprim (OMP) in Nile tilapia (Oreochromis sp.) after oral administration aiming to estimate a minimum withdrawal. The medicated feed used was prepared and analyzed to ensure suitability for animal treatment and prevent environmental contamination. After treatment, fish were euthanized at different times, their fillets were collected, and the drug residues were determined. Two analytical methods were developed to quantify SDM and OMP in feed and fillet samples. Meanwhile, a withdrawal period of 252 & DEG;C days was estimated. Sulfadimethoxine (SDM) and ormetoprim (OMP) are antimicrobials used in combination to treat bacterial infections in fish farming. The use of this drug combination is not yet regulated in some countries, such as Brazil. Due to the lack of regulated drugs for aquaculture in Brazil, this study investigated the residue depletion profile of SDM and OMP in Nile tilapia (Oreochromis sp.) after oral administration. Fish were treated with medicated feed containing a 5:1 ratio of SDM:OMP at the dose of 50 mg kg BW-1 for five consecutive days with an average water temperature of 28 & DEG;C. The drugs were incorporated into the feed by using a gelatin coating process which promoted homogeneity in drug concentration and prevented the drug leaching into the water during medication. The SDM and OMP determination in fish fillets (muscle plus skin in natural proportions) was performed using the QuEChERS approach followed by LC-MS/MS quantification. The analytical method was validated according to Brazilian and selected international guidelines. A withdrawal period of 9 days (or 252 & DEG;C days) was estimated for the sum of SDM and OMP residues at concentration levels below the maximum residue level of 100 & mu;g kg(-1). (AU)

• FAPESP's process: 21/03294-1 - Studies for the use of levamisole in Aquaculture: analytical methods; incorporation in the feed; residual depletion; and withdrawal period estimate
• Grantee: Lucas Victor Pereira de Freitas
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/06159-8 - Studies for the safe use of erythromycin and doxycycline in fish of national commercial importance
• Grantee: Jonas Augusto Rizzato Paschoal
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/16593-9 - Estimation and evaluation of withdrawal periods for albendazole and its metabolites in fish
• Grantee: Ana Carolina Vellosa Portela
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/05536-0 - Erythromycin in aquaculture: studies for drug incorporation in feed and for residual depletion in fish
• Grantee: Sarah Chagas Campanharo
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 21/08152-0 - Challenge of the use of drugs in fish farming in the context of One Health: evaluation of efficacy, safety and risk analysis
• Grantee: Susanne Rath
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/22775-0 - Combination of thiamphenicol with florfenicol: pharmacokinetics and therapeutic efficacy in the treatment of Streptococcosis in Nile Tilapia (Oreochromis niloticus)
• Grantee: Inácio Mateus Assane
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/18334-0 - Veterinary drug residues in food: multiclass analytical methods employing LC-MS/MS, exposition data and residual depletion study
• Grantee: Jonas Augusto Rizzato Paschoal
• Support Opportunities: Research Grants - Young Investigators Grants