Electrochemical magneto-immunoassay for detection of ADAM10 Alzheimer's biomarker using gold nanoparticles as label

Author(s)

de Oliveira, Tassia R. ; Manzine, Patricia R. ; Cominetti, Marcia R. ; Leite, Oldair D. ; Faria, Ronaldo C.

• Document type: Journal article
• Source: Talanta; v. 266, p. 8-pg., 2024-01-01.

Abstract

Alzheimer's disease (AD), a neurodegenerative and progressive illness with no known cure, is the most frequent cause of dementia in older adults. Dementia in AD is usually preceded by a stage of cognitive decline known as mild cognitive impairment (MCI). MCI has gained attention as an ideal target for prevention and early interventions, considering its reversible characteristic. Here, we propose a magneto-immunoassay based on a lowcost screen-printed electrode for detecting soluble ADAM10 in plasma samples, a potential biomarker for early AD diagnosis. We present a sandwich immunoassay using magnetic beads modified with antibodies to capture ADAM10 from plasma samples and using gold nanoparticles (AuNPs) as an electrochemical label. The assay was designed to accurately detect ADAM10 in diluted plasma with a limit of detection (LoD) of 32.5 pg/mL and a dynamic linear range of 10.0-1000.0 pg/mL. Twenty-three plasma samples from the elderly, including patients with AD, MCI, and healthy subjects (negative control), were analyzed by the magneto-immunoassay and enzymelinked immunosorbent assay (ELISA), and the ADAM10 levels correlated. This work shows the potential of this protein as a biomarker in the early diagnosis and progression of AD and provides an interesting disposable device with capabilities for applications as point-of-care (PoC) to measure ADAM10 levels. (AU)

• FAPESP's process: 17/24053-7 - Development of simple electrochemical methods for the detection of biomarkers for the diagnosis of diseases
• Grantee: Ronaldo Censi Faria
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/26084-1 - Evaluation of platelet ADAM10 in non-Alzheimer's dementias
• Grantee: Patricia Regina Manzine Moralles
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/24839-0 - Nanoelectronics and nanoscale electrochemistry: fundaments and applications
• Grantee: Paulo Roberto Bueno
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/19890-1 - Development of methods for diagnosis of diseases using magnetic particles and an array of imunossensors in a disposable microfluidic system
• Grantee: Ronaldo Censi Faria
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/01863-9 - Biology and function of ADAM10 isoforms for differential diagnosis of Alzheimer's Disease by electrochemical sensors
• Grantee: Márcia Regina Cominetti
• Support Opportunities: Research Projects - Thematic Grants