The effect of ultrasound on improving the extraction of tannins from the Stryphnodendron adstringens bark

Author(s)

Ribeiro, Mauricio M. de Souza ; Vigano, Juliane ; de Novais, Nathalia Stahl ; Mesquita, Leonardo M. de Souza ; Kamikawachi, Renan Canute ; Vilegas, Wagner ; Lopes, Patricia Santos ; da Silva, Camila Soares ; Rostagno, Mauricio Ariel ; Veggi, Priscila Carvalho

• Document type: Journal article
• Source: SUSTAINABLE CHEMISTRY AND PHARMACY; v. 33, p. 14-pg., 2023-03-10.

Abstract

Stryphnodendron adstringens (Barbatimao) barks is one of the plants listed in the national list of medicinal plants of interest to the Brazilian health system and in the Brazilian pharmacopeia. Its extracts have been used in commercial products and popular medicine due to their phenolic compound's composition. Reported works dealing with Barbatimao and ultrasound-assisted extraction (UAE) does not cover all possible variables to be studied. Also, there is a gap in understanding the ultrasound phenomena's effect on the extract composition and biological properties of the Barbatimao. This work aimed to apply the UAE, using hydroethanolic solution (50 wt%) as the solvent to recover phenolic compounds from Barbatimao barks. Amplitude (0-40%), solvent-to-feed mass ratio (5-25), and extraction time (2-12 min) were evaluated. Ferric-reducing antioxidant capacity (FRAP), antioxidant cell activity, and cytotoxicity were also evaluated. The results showed that UAE parameters play significant roles in obtaining higher yields of phenolic compounds compared to other processes including conventional techniques. It was identified that the ultrasound waves contribute to enhancing the tannin extraction through mechanisms that only ultrasound, among the tested techniques, can provide. The extract obtained by UAE promoted cell viability higher than 80%, while the extracts obtained by the conventional method did not demonstrate this activity. UAE showed an efficient technique to obtain a tannins-rich extract from Barbatimaobarks. (AU)

• FAPESP's process: 20/15774-5 - Development of methods of extraction, separation and two-dimensional analysis of phenolic compounds in natural products
• Grantee: Juliane Viganó
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 19/13496-0 - Multi user equipment approved in grant 2018/14582-5: ultra-high pressure liquid chromatography system (UHPLC), composed by a quaternary pump, autoinjector, oven with selection valve and detector (PDA)
• Grantee: Maurício Ariel Rostagno
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 20/07575-2 - Application of high pressure technology to obtain bioproducts: assembly of a multi-purpose equipment and development of processes for barbatimão
• Grantee: Priscilla Carvalho Veggi
• Support Opportunities: Regular Research Grants