| Full text | |
| Author(s): |
Mantovani, Raphaela Araujo
;
Hamon, Pascaline
;
Rousseau, Florence
;
Tavares, Guilherme M.
;
Mercadante, Adriana Zerlotti
;
Croguennec, Thomas
;
Bouhallab, Said
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Food Research International; v. 138, p. 9-pg., 2020-12-01. |
| Abstract | |
Understanding the food protein binding to bioactive compounds is of utmost importance for the development of efficient protein-based delivery systems. The binding of lutein to sodium caseinate (NaCas) or native casein micelle (PPCN) was investigated at pH 7 to evaluate the effect of casein supramolecular structures on the interaction. Fluorescence quenching, UV-vis spectroscopy, and dynamic light scattering were carried out. Under the medium conditions of interaction analysis (DMSO-water and ethanol-water), lutein exists as H-type aggregates. The investigation of lutein/casein interaction showed a predominantly static mechanism of fluorescence quenching and the presence of two fluorophore populations on NaCas and PPCN, but only one accessible to lutein. Moreover, the Scatchard plot indicated that lutein interacted with both caseins in one binding site. The interaction of lutein with caseins occurred with binding constant K-b of 10(5) M-1, regardless of casein supramolecular structure. (AU) | |
| FAPESP's process: | 18/18374-8 - Understanding interactions between caseins and carotenoids at a molecular level |
| Grantee: | Raphaela de Araujo Mantovani |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 17/26237-8 - Understanding how protein-carotenoid interactions influence emulsion stability and carotenoid bioacessibility |
| Grantee: | Raphaela de Araujo Mantovani |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 17/09214-4 - How do structural changes of dairy proteins affect the functionality of bioactive compounds in complex food matrices? |
| Grantee: | Guilherme Miranda Tavares |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 13/07914-8 - FoRC - Food Research Center |
| Grantee: | Bernadette Dora Gombossy de Melo Franco |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |