Chitosan films activated with phytochemicals extracted from carrot by-product encapsulated in TPP chitosomes

Author(s)

Esposto, Bruno Stefani ; Tessaro, Larissa ; Aguilar, Guilherme Jose ; Sobral, Paulo Jose do Amaral ; Tapia-Blacido, Delia Rita ; Martelli-Tosi, Milena

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY; v. 59, n. 3, p. 10-pg., 2023-12-26.

Abstract

The main objective of this study was to extract phytochemicals from carrot by-product, encapsulate them in TPP chitosomes (consisting of liposome vesicles coated with chitosan cross-linked with sodium tripolyphosphate) and incorporate them into chitosan (Ch) films. Physicochemical, antioxidant and antimicrobial properties of films were evaluated. Three concentrations of TPP chitosome suspension were studied: 50%, 60% and 70%, designated TPPCh50, TPPCh60 and TPPCh70 respectively. Microscopy analyses showed that the particles modified the surface and internal structures of the Ch films, providing films with higher average roughness (Control: 12.6 +/- 1.8 nm vs. TPPCh70: 96.1 +/- 8.0 nm) and decreased gloss (Control: 34.1 +/- 3.9 GU vs. TPPCh70: 5.6 +/- 1.0 GU). Furthermore, the TPPCh films displayed decreased Ch intermolecular interactions, which produced less mechanically resistant (TS = 15.3 MPa), more elongable (EB = 47.9%), slightly more water-soluble (SI = 14.7%) and more water-vapour permeable (WVP = 7.54*10(-9) g/msPa) films, mainly at high phytochemical concentration (TPPCh70) as compared to the Control. TPPCh addition in the Ch film improved its antioxidant capacity (3.6 times greater) and UV/Vis light barrier property, but no significant antimicrobial activity was observed. In conclusion, TPPCh films could be used as packaging to preserve foods that are sensitive to oxidation. (AU)

• FAPESP's process: 19/23171-1 - Byproducts recovery from food processing: extraction of phytochemical compounds, encapsulation and application in biodegradable films / coatings
• Grantee: Milena Martelli Tosi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/18788-1 - Raman microspectroscopy and chemometrics for the characterization of polymer-based microcarrier systems of active/photoactive compounds
• Grantee: Milena Martelli Tosi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/22502-4 - Study of in vitro digestion of TPP-chitosomes encapsulating phytochemical compounds extracted from carrot byproducts
• Grantee: Bruno Stefani Esposto
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 19/05511-0 - Byproducts recovery from carrot juice processing: extraction of phytochemical compounds, encapsulation and application in biodegradable films
• Grantee: Bruno Stefani Esposto
• Support Opportunities: Scholarships in Brazil - Master