| Full text | |
| Author(s): Show less - |
de Lima, Lucas F.
;
Corsato, Paula C. R.
;
Beluomini, Maisa A.
;
Ferreira, Andre L.
;
Santos, Leticia Esterdos
;
Barbosa, Priscilla P.
;
Simeoni, Camila L.
;
de Jesus, Marcelo Bispo
;
Proenca-Modena, Jose Luiz
;
Paixao, Thiago R. L. C.
;
de Araujo, William R.
Total Authors: 11
|
| Document type: | Journal article |
| Source: | ACS APPLIED ELECTRONIC MATERIALS; v. 7, n. 7, p. 12-pg., 2025-03-21. |
| Abstract | |
The latest outbreak caused by monkeypox virus (MPXV) has turned into an international public health emergency, underscoring the urgent need for rapid, large-scale, and sensitive diagnostic tests for MPXV. Here, a capacitive biosensor for detecting MPXV was developed using a laser-scribed graphene (LSG) sensor manufactured on synthetic aramid fiber. The aramid-LSG sensor was modified with monoclonal antibodies for detecting MPXV through electrochemical capacitance measurements (C mu -). The electrochemical detection was performed using a system of two interdigitated electrodes, providing excellent reproducibility and without cross-reactivity in the presence of other poxviruses and nonpoxviruses. Also, the wearable textile biosensor achieved a LOD of 7.5 x 10-1 PFU mL-1 and a LOQ of 2.4 x 100 PFU mL-1, enabling its application in plasma, saliva, and PBS samples (simulating application to human skin containing the virus). Furthermore, cytotoxicity assay studies demonstrated that the device is safe to use, according to the in vitro studies employing 3T3 cell cultures. This approach demonstrates the great potential of the wearable capacitive biosensor, which can be manufactured on a large scale using an environmentally friendly method for the wearable analysis of MPXV on patient skin. (AU) | |
| FAPESP's process: | 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications |
| Grantee: | Mauro Bertotti |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 22/03250-7 - Development of portable and cost-effective biosensors for clinical diagnosis of bacterial and viral infections |
| Grantee: | William Reis de Araujo |
| Support Opportunities: | Research Grants - Initial Project |
| FAPESP's process: | 23/00246-1 - Miniaturized large-scale devices for in-situ analysis: fabrication, characterization and applications |
| Grantee: | Mauro Bertotti |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 22/10442-0 - PAMPA network to assess the impact of environmental degradation on the dynamics of circulation of emerging and reemerging viruses in the Amazon region |
| Grantee: | José Luiz Proença Módena |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 22/00723-1 - The role of IRF5 in the humoral response in emerging arboviroses with acute or chronic manifestations |
| Grantee: | Camila Lopes Simeoni |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 23/12589-0 - Fabrication of multiplex electrochemical (bio)sensors by CO2 laser engraving aiming the development of wearable devices. |
| Grantee: | Lucas Felipe de Lima |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |