Effect of colonic fermentation of uvaia (Eugenia pyriformis Cambess) fractions on phenolic compounds and gut microbiota composition: An vitro study using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)

Author(s)

Farias, David de Paulo ; de Araujo, Fabio Fernandes ; Huyan, Zongyao ; Villasante, Juliana ; Ranpatabendi, Thisun ; Fogliano, Vincenzo ; Pastore, Glaucia Maria

• Document type: Journal article
• Source: Food Chemistry; v. 488, p. 11-pg., 2025-10-01.

Abstract

This study evaluated the effect of colonic fermentation of uvaia seed and uvaia pulp on the phenolic compounds produced in the lumen and on the gut microbiota composition. Fermentation of uvaia pulp increased epigallocatechin, hippuric acid, ellagic acid, epicatechin gallate, and procyanidins in proximal colon (PC). The microbiota action on uvaia seed decreased hesperetin and quercetin in descending colon (DC). Fecal fermentation of uvaia pulp fraction showed the highest production of acetate (1469.82-5860.10 mg/L), propionate (543.72-3039.37 mg/L), and butyrate (8.58-2225 mg/L). Firmicutes decreased its overabundance after fermentation of both uvaia fractions. Feeding with uvaia seed reduced Veillonella in all the colon compartments and increased Akkermansia in DC. These results suggest that uvaia consumption can increase polyphenol concentrations in the gut lumen, primarily through the release of bound phenolic compounds from the plant cell wall during colonic fermentation, which in turn modifies the composition of the gut microbiota. (AU)

• FAPESP's process: 22/04530-3 - Antidiabetic potential of xique-xique (Pilosocereus gounellei) and mandacaru (Cereus jamacaru) fruits after colonic fermentation
• Grantee: Fábio Fernandes de Araújo
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 22/05242-1 - Effect of colonic fermentation on the antidiabetic potential of uvaia (Eugenia pyriformis Cambess.)
• Grantee: David de Paulo Farias
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 20/00225-6 - Determination of antioxidant potential, in vitro bioaccessibility and evaluation of antidiabetic and prebiotic activities from the uvaia (Eugenia pyriformis Cambess)
• Grantee: David de Paulo Farias
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/50333-1 - COBRA: a novel compound discovery pipeline for rapid and cost-effective identification of bioactives with health beneficial effects in Brazilian crops
• Grantee: Glaucia Maria Pastore
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/15163-6 - Determination of chemical composition, bioaccessibility and evaluation of antidiabetic and prebiotic activities from Xique-Xique (Pilosocereus gounellei) and Mandacaru (Cereus jamacaru) fruits
• Grantee: Fábio Fernandes de Araújo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral