Biosensor Based on Electrocodeposition of Carbon Nanotubes/Polypyrrole/Laccase for Neurotransmitter Detection

Author(s)

Cesarino, Ivana ^[1] ; Galesco, Heloisa V. ^[1] ; Moraes, Fernando C. ^[1] ; Lanza, Marcos R. V. ^[1] ; Machado, Sergio A. S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Electroanalysis; v. 25, n. 2, p. 394-400, FEB 2013.
• Web of Science Citations: 20

Abstract

A new biosensor has been developed by a simple electrocodeposition of multiwalled carbon nanotubes (MWCNT), polypyrrole (PPy) and laccase (Lac) on the platinum (Pt) electrode surface. The neurotransmitter biosensor was applied to the detection of dopamine in urine samples using differential pulse voltammetry (DPV). The Pt/MWCNT/PPy/Lac biosensor exhibited a detection limit of 0.14 mu M, which is an adequate level for monitoring dopamine in urine. Reproducibility and repeatability values of 2.9% and 1.7%, respectively, were obtained compared to the conventional procedure. The proposed biosensor was successfully applied in the determination of dopamine in urine, and the obtained results were in full agreement with those from the HPLC procedure. (AU)

• FAPESP's process: 10/11049-2 - Development and applications of electrochemical sensors based on aligned carbon nanotubes for endocrine disrupting detection in environmental samples
• Grantee: Fernando Cruz de Moraes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 10/11567-3 - On-site analysis of the products formed in the electrochemical degradation of monoaromatic hydrocarbons
• Grantee: Ivana Cesarino
• Support Opportunities: Scholarships in Brazil - Post-Doctoral