DEVELOPMENT OF SCAFFOLDS FOR ODONTOLOGICAL APPLICATIONS

• Grant number: 18/13432-0
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2018
• End date: September 30, 2021
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Norberto Aranha
• Grantee: Norberto Aranha
• Host Institution: Pró-Reitoria de Pós-Graduação, Pesquisa, Extensão e Inovação. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil
• Associated researchers: Angela Faustino Jozala ; Denise Grotto ; Karina Gonzales Silvério Ruiz ; Marco Vinícius Chaud

Abstract

Scaffolds of biopolymers of natural, synthetic or composite origin will be produced and evaluated, aiming their application in the regeneration of the support alveolar bone. These biopolymers must form a three-dimensional, highly porous, resistant, resilient and bioadhesive framework. Scaffolds will be obtained from three-dimensional structured hydrogels with the possibility of being molded in lamellar, laminated, cylindrical, cubic, granulated and so on. Scaffolds will be designed for a bio-persistence of at least 90 days. To achieve this retention time, the formulations will be composed of cross-linked biopolymers capable of regulating biodegradation and maintaining physiological functions and stimulating the regeneration of bone tissue. The biopolymers to be used will be collagen type I (COL-I), silk fibroin (FS), bacterial nano-cellulose (nCB), chitosan (Qt), hyaluronic acid (HA) and sodium alginate (AS). Curcumin (CUR), due to its anticancer and antibacterial action, and hydroxyapatite (Hap) incorporation to the scaffolds will be tested to increase surface reactivity, expanding integration with the adjacent tissue stimulating adhesion, migration and proliferation, in addition to improving mechanical resistance. The samples will be characterized as their physicochemical, physicomechanical, porosity and CUR release. In vitro tests will be performed with pre-osteoblastic mouse cells for mineralization, gene expression, cell viability, alkaline phosphatase activity, MTT assay and scanning electron microscopy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VICTÓRIA SOARES SOEIRO; LOUISE LACALENDOLA TUNDISI; VENÂNCIO ALVES AMARAL; FENANDO BATAIN; PRISCILA GAVA MAZZOLA; ELIAS BASILE TAMBOURGI; JOSÉ MARTINS DE OLIVEIRA JÚNIOR; MARCO VINICIUS CHAUD; DENISE GROTTO; NORBERTO ARANHA; et al. Nanocelulose bacteriana e fibroína: produtos naturais para a produção de membranas estruturais. MATERIA-RIO DE JANEIRO, v. 26, n. 4, 2022-01-05. (18/13432-0, 16/05930-4)

ALVES, THAIS F. R.; RIOS, ALESSSANDRA C.; PONTES, KATIUSCA DA SILVA; PORTELLA, DECIO L.; ARANHA, NORBERTO; SEVERINO, PATRICIA; SOUTO, ELIANA B.; GONSALVES, JOYCE K. M.; NUNES, ROGERIA DE SOUZA; CHAUD, V, MARCO. Bilayer Mucoadhesive Buccal Film for Mucosal Ulcers Treatment: Development, Characterization, and Single Study Case. PHARMACEUTICS, v. 12, n. 7, JUL 2020. (11/21219-5, 18/13432-0, 18/11350-6)

AMARAL, VENANCIO ALVES; RIBEIRO ALVES, THAIS FRANCINE; DE SOUZA, JULIANA FERREIRA; BATAIN, FERNANDO; DE MOURA CRESCENCIO, KESSI MARIE; SOEIRO, VICTORIA SOARES; DE BARROS, CECILIA TORQUETI; CHAUD, MARCO VINICIUS. Phenolic Compounds from Psidium guajava (Linn.) Leaves: Effect of the Extraction-Assisted Method Upon Total Phenolics Content and Antioxidant Activity. BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY, v. 11, n. 2, p. 12-pg., 2021-04-15. (18/13432-0, 11/21219-5)