Construction of a pediocin-producing recombinant lineage from agroindustrial waste

• Grant number: 18/04385-8
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2018
• End date: November 30, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Ricardo Pinheiro de Souza Oliveira
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Marcio Vinicius Bertacine Dias ; Paul Cotter ; Taís Mayumi Kuniyoshi ; Welington Luiz de Araújo
• Associated research grant(s): 19/23585-0 - Towards internationalization of Agrobiotechnology Research between University of São Paulo (USP), Brazil, and University of Natural Resources and Life Sciences (BOKU), Austria, AP.R SPRINT

Abstract

A significant effort is underway to develop new antimicrobial compounds for both food and pharmaceutical industry. Bacteriocins are peptides produced by a number of bacteria that harbor bacteriostatic or bactericide effect over pathogenic and deleterious microorganism present in industrial processes. The ones produced by lactic acid bacteria (LABs) present an interest on industry as some of them are probiotic, therefore, acknowledged as safe on this field. In this context, the aim of this project is the maximization of pediocin A production by Pediococcus pentosaceus ET 34 probiotic bacterium through molecular biology tools combined with techniques of scale up improvement on fermentation process. The first step of this project consists in the identification pediocin gene from P. pentosaceus ET 34 and the physical chemical characterization of this bacteriocin. Secondly, the construction of a recombinant strain of P. pentosaceus ET 34 over-expressing heterologously the induction factor and subsequently the comparison of its pediocin productivity with the corresponding wild type via qPCR in order to quantify pediocin transcripts. Finally, we intend to evaluate the effect of different parameters on pediocin productivity in the recombinant strain of P. pentosaceus ET 34. For this purpose, the experiments will be carry out using fractional factorial designs and/or rotational central composite design (RCCD) using agroindustrial waste, such as hydrolyzed sugarcane bagasse with high xylooligosaccharide content. The results obtained will lead to establish the scale up parameters, starting from small scale assays followed by 3L discontinuous bioreactor. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ALMEIDA, GABRIEL MORETTI; NOBREGA MENDONCA, CARLOS MIGUEL; CONVERTI, ATTILIO; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. Kinetic and thermodynamic parameters of nisin thermoinactivation. Journal of Food Engineering, v. 280, SEP 2020. (19/19054-0, 18/04385-8)

KUNIYOSHI, TAIS MAYUMI; NOBREGA MENDONCA, CARLOS MIGUEL; VIEIRA, VIVIANE BORGES; ROBL, DIOGO; GOMBOSSY DE MELO FRANCO, BERNADETTE DORA; TODOROV, SVETOSLAV DIMITROV; TOME, ELISABETTA; O'CONNOR, PAULA MARY; CONVERTI, ATTILIO; ARAUJO, WELINGTON LUIZ; et al. Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse. APPLIED GEOCHEMISTRY, v. 131, AUG 2021. (18/04385-8, 18/25511-1, 15/24777-0, 13/07914-8)

KUNIYOSHI, TAIS MAYUMI; NOBREGA MENDONCA, CARLOS MIGUEL; VIEIRA, VIVIANE BORGES; ROBL, DIOGO; GOMBOSSY DE MELO FRANCO, BERNADETTE DORA; TODOROV, SVETOSLAV DIMITROV; TOME, ELISABETTA; O'CONNOR, PAULA MARY; CONVERTI, ATTILIO; ARAUJO, WELINGTON LUIZ; et al. ediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagass. Bioresource Technology, v. 338, OCT 2021. (13/07914-8, 18/04385-8, 15/24777-0, 18/25511-1)

DE AZEVEDO, PAMELA O. S.; MENDONCA, CARLOS M. N.; SEIBERT, LIANE; DOMINGUEZ, JOSE M.; CONVERTI, ATTILIO; GIERUS, MARTIN; OLIVEIRA, RICARDO P. S.. Bacteriocin-like inhibitory substance of Pediococcus pentosaceus as a biopreservative for Listeria sp. control in ready-to-eat pork ham. Brazilian Journal of Microbiology, v. 51, n. 3, p. 949-956, SEP 2020. (18/04385-8)

O'CONNOR, PAULA M.; KUNIYOSHI, TAIS M.; OLIVEIRA, RICARDO P. S.; HILL, COLIN; ROSS, REYNOLDS PAUL; COTTER, PAUL D.. Antimicrobials for food and feed; a bacteriocin perspective. CURRENT OPINION IN BIOTECHNOLOGY, v. 61, p. 160-167, FEB 2020. (18/04385-8, 15/24777-0, 18/02519-7)

DE SOUZA DE AZEVEDO, PAMELA OLIVEIRA; DE AZEVEDO, HERNANDO FERNANDES; FIGUEROA, ELIAS; CONVERTI, ATTILIO; MANUEL DOMINGUEZ, JOSE; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. Effects of pH and sugar supplements on bacteriocin-like inhibitory substance production by Pediococcus pentosaceus. MOLECULAR BIOLOGY REPORTS, v. 46, n. 5, p. 4883-4891, OCT 2019. (18/04563-3, 18/04385-8)

DE SOUZA DE AZEVEDO, PAMELA OLIVEIRA; NOBREGA MENDONCA, CARLOS MIGUEL; RAMOS MORENO, ANA CAROLINA; IANK BUENO, ANTONIO VINICIUS; YOKOMIZO DE ALMEIDA, SONIA REGINA; SEIBERT, LIANE; CONVERTI, ATTILIO; WATANABE, II-SEI; GIERUS, MARTIN; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. Antibacterial and antifungal activity of crude and freeze-dried bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus. SCIENTIFIC REPORTS, v. 10, n. 1, JUL 23 2020. (18/25511-1, 18/04385-8)