Development and application of nuclear magnetic resonance in products and agricultural processes

• Grant number: 19/13656-8
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2020
• End date: March 31, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Luiz Alberto Colnago
• Grantee: Luiz Alberto Colnago
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated scholarship(s): 21/03853-0 - Applications of 100 mm magnetic-based TD-NMR spectrometer on products of agricultural interest, BP.TT; 20/10553-0 - Development and validation of time domain NMR instrumentation for analysis of agricultural products, BP.TT; 20/07017-0 - Development of non-destructive methods for assessing the quality of intact food by NMR in the time domain, BP.TT

Abstract

Agribusiness is one of the main sectors of the Brazilian economy and not only supplies the domestic market but is also the country's main export sector. Despite this great importance, agricultural products are exported as commodities which indicates that they are exported without any quality specification. In order to be marketed in a differentiated way it is necessary to have fast quality determination and certification processes. This requires the development of rapid and non-destructive methods that can be used, if necessary, in the analysis of all agricultural products. Among the techniques that have this potential is the time domain nuclear magnetic resonance spectroscopy (TD-NMR), which use low field permanent magnets. Using this instruemnts we have developed dozens of analytical methods that are being incorporated into the productive sector through a partnership with the national industry. In addition, we developed NMR methods to monitor electrochemical reactions in situ and studies of metal corrosion, which is one of the main problems of reducing the life of agricultural machinery. Most of these methods were developed with FAPESP resources for research assistance, master's, doctoral and postdoctoral fellowships. We have also used high resolution NMR (HR-NMR) in agricultural research for the study of animal, plant and microorganism physiology. With these studies it has been possible to study the development and productivity of animals and plants, and to determine the effects of water stress on sensitive and tolerant plants. Thus, the aim of this research project will be to continue to develop new TD-NMR methods and instrumentation for analysis of fresh and processed foods, as well as using the HR-NMR to study plant metabolome under abiotic stress, food and other agricultural systems. (AU)

Scientific publications

(29)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONSOLO, NARA R. B.; SILVA, JULIANA; BUARQUE, VICENTE M.; BARBOSA, LUIS C.; PADILLA, ANGEL H.; COLNAGO, LUIZ A.; NETTO, ARLINDO SARAN; GERRARD, DAVID E.; SILVA, SAULO L.. Metabolomic signature of genetic potential for muscularity in beef cattle. ANIMAL BIOTECHNOLOGY, MAY 2021. (16/17433-5, 19/13656-8, 16/01320-7)

MONARETTO, TATIANA; MONTRAZI, ELTON TADEU; MORAES, TIAGO BUENO; SOUZA, ANDRE ALVES; RONDEAU-MOURO, CORINNE; COLNAGO, LUIZ ALBERTO. Using T-1 as a direct detection dimension in two-dimensional time-domain NMR experiments using CWFP regime. JOURNAL OF MAGNETIC RESONANCE, v. 311, FEB 2020. (19/13656-8, 17/12864-0)

FACCHINATTO, WILLIAM MARCONDES; DOS SANTOS, DANILO MARTINS; FIAMINGO, ANDERSON; BERNARDES-FILHO, RUBENS; CAMPANA-FILHO, SERGIO PAULO; DE AZEVEDO, EDUARDO RIBEIRO; COLNAGO, LUIZ ALBERTO. Evaluation of chitosan crystallinity: A high-resolution solid-state NMR spectroscopy approach. Carbohydrate Polymers, v. 250, DEC 15 2020. (17/24465-3, 16/20970-2, 17/20973-4, 16/09720-4, 19/13656-8)

DA SILVA, POLLYANA FERREIRA; GOMES, BRUNA FERREIRA; SILVA LOBO, CARLOS MANUEL; CARMO, MARCELO; ROTH, CHRISTINA; COLNAGO, LUIZ ALBERTO. Composite Graphite-Epoxy Electrodes for In Situ Electrochemistry Coupling with High Resolution NMR. ACS OMEGA, v. 7, n. 6, p. 10-pg., 2022-01-31. (21/12694-3, 19/13656-8, 12/22281-9)

FACCHINATTO, WILLIAM MARCONDES; GARCIA, RODRIGO HENRIQUE DOS SANTOS; SANTOS, DANILO MARTINS DOS; FIAMINGO, ANDERSON; FLORES, DOUGLAS WILLIAM MENEZES; CAMPANA-FILHO, SERGIO PAULO; AZEVEDO, EDUARDO RIBEIRO DE; COLNAGO, LUIZ ALBERTO. Fast-forward approach of time-domain NMR relaxometry for solid-state chemistry of chitosan. Carbohydrate Polymers, v. 256, MAR 15 2021. (16/09720-4, 17/20973-4, 19/13656-8)

COLNAGO, LUIZ ALBERTO; WIESMAN, ZEEV; PAGES, GUILHEM; MUSSE, MAJA; MONARETTO, TATIANA; WINDT, CAREL W.; RONDEAU-MOURO, CORINNE. Low field, time domain NMR in the agriculture and agrifood sectors: An overview of applications in plants, foods and biofuels. JOURNAL OF MAGNETIC RESONANCE, v. 323, FEB 2021. (19/13656-8, 20/07017-0)

FLÁVIO V. C. KOCK; LUIZ A. COLNAGO. NMR Relaxometry Applied to Chemical Studies of Paramagnetic Metal Cation Complexes: Fundamentals and Applications. Journal of the Brazilian Chemical Society, v. 33, n. 6, p. 509-522, 2022-05-30. (12/23169-8, 19/13656-8, 18/16040-5, 21/12694-3, 15/16624-9)

MONTRAZI, ELTON TADEU; MONARETTO, TATIANA; BONAGAMBA, TITO JOSE; COLNAGO, LUIZ ALBERTO. New and rapid pulse sequences for two-dimensional D-T-1 correlation measurements. JOURNAL OF MAGNETIC RESONANCE, v. 315, JUN 2020. (19/13656-8, 09/54880-6, 17/12864-0, 12/00380-5)

ALENCAR OLDONI, FERNANDA CAMPOS; FLORENCIO, CAMILA; BERTAZZO, GIOVANA BRAIT; GRIZOTTO, PAMELA APARECIDA; BOGUSZ JUNIOR, STANISLAU; CARNEIRO, RENATO LAJARIM; COLNAGO, LUIZ ALBERTO; FERREIRA, MARCOS DAVID. Fruit quality parameters and volatile compounds from 'Palmer' mangoes with internal breakdown. Food Chemistry, v. 388, p. 9-pg., 2022-09-15. (19/13656-8, 19/05159-4)

YVES JOSÉ DE SOUZA SANTOS; NATALIA ALVES CAMPOS; GERSON BALBUENO BICCA; GISELE TEIXEIRA DE SOUZA SORA; LUIS FERNANDO POLESI; TANIA MARIA ALBERTE; POLIANA M. SANTOS; RODRIGO HENRIQUE DOS SANTOS GARCIA; LUIZ ALBERTO COLNAGO; GABRIELI OLIVEIRA-FOLADOR. Non-destructive determination of the oil content in peach palm (Bactris gasipaes) flour using NMR and NIR spectroscopies. FOOD SCIENCE AND TECHNOLOGY, v. 43, 2023-01-16. (19/13656-8, 21/12694-3)

BRANDAO CONSOLO, NARA REGINA; ROSA, ALESSANDRA FERNANDES; GARIBALDI SIMON BARBOSA, LUIS CARLOS; MACLEAN, PAUL HAYDON; HIGUERA-PADILLA, ANGEL; COLNAGO, LUIZ ALBERTO; LENCIONI TITTO, EVALDO ANTONIO. Preliminary study on the characterization of Longissimus lumborum dark cutting meat in Angus x Nellore crossbreed cattle using NMR-based metabolomics. MEAT SCIENCE, v. 172, FEB 2021. (19/13656-8, 18/09800-3)

CONSOLO, NARA R. B.; SAMUELSSON, LINDA M.; BARBOSA, LUIS C. G. S.; MONARETTO, TATIANA; MORAES, TIAGO B.; BUARQUE, VICENTE L. M.; HIGUERA-PADILLA, ANGEL R.; COLNAGO, LUIZ A.; SILVA, SAULO L.; REIS, MARLON M.; et al. Characterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry: a pilot study. POULTRY SCIENCE, v. 99, n. 11, p. 6247-6257, NOV 2020. (18/09800-3, 19/13656-8)

GARCIA, RODRIGO HENRIQUE DOS SANTOS; FILGUEIRAS, JEFFERSON GONCALVES; COLNAGO, LUIZ ALBERTO; DE AZEVEDO, EDUARDO RIBEIRO. eal-Time Monitoring Polymerization Reactions Using Dipolar Echoes in H-1 Time Domain NMR at a Low Magnetic Fiel. Molecules, v. 27, n. 2, JAN 2022. (19/13656-8, 17/24465-3, 21/12694-3)

MONARETTO, TATIANA; MORAES, TIAGO BUENO; COLNAGO, LUIZ ALBERTO. Recent 1D and 2D TD-NMR Pulse Sequences for Plant Science. PLANTS-BASEL, v. 10, n. 5, MAY 2021. (19/13656-8, 20/07017-0)

HIGUERA-PADILLA, ANGEL RUBEN; KOCK, FLAVIO VINICIUS CRIZOSTOMO; BATISTA, ALZIR AZEVEDO; COLNAGO, LUIZ ALBERTO. A straightforward catalytic approach to obtain deuterated chloroform at room temperature. Magnetic Resonance in Chemistry, v. 58, n. 10, JUL 2020. (12/23169-8, 19/13656-8, 17/02197-7, 18/16040-5)

NASCIMENTO MITRE, CIRLEI IGREJA; GOMES, BRUNA FERREIRA; PARIS, ELAINE; SILVA LOBO, CARLOS MANUEL; ROTH, CHRISTINA; COLNAGO, LUIZ ALBERTO. Use of Time Domain Nuclear Magnetic Resonance Relaxometry to Monitor the Effect of Magnetic Field on the Copper Corrosion Rate in Real Time. MAGNETOCHEMISTRY, v. 8, n. 4, p. 10-pg., 2022-04-01. (21/12694-3, 19/13656-8, 12/22281-9)

CONSOLO, N. R. B.; BUARQUE, V. L. M.; SILVA, J.; POLETI, M. D.; BARBOSA, L. C. G. S.; HIGUERA-PADILLA, A.; GOMEZ, J. F. M.; COLNAGO, L. A.; GERRARD, D. E.; NETTO, A. SARAN; et al. Muscle and liver metabolomic signatures associated with residual feed intake in Nellore cattle. ANIMAL FEED SCIENCE AND TECHNOLOGY, v. 271, JAN 2021. (19/13656-8, 16/01320-7, 16/17433-5, 18/09800-3)

NASCIMENTO MITRE, CIRLEI IGREJA; TOSIN, GIANCARLO; COLNAGO, LUIZ ALBERTO. In-operando analysis of the corrosion patterns and rates under magnetic fields using metallic film. NPJ MATERIALS DEGRADATION, v. 6, n. 1, p. 7-pg., 2022-03-24. (21/12694-3, 19/13656-8)

FACCHINATTO, WILLIAM MARCONDES; DOS SANTOS, DANILO MARTINS; BUKZEM, ANDREA DE LACERDA; MORAES, TIAGO BUENO; HABITZREUTER, FILIPE; DE AZEVEDO, EDUARDO RIBEIRO; COLNAGO, LUIZ ALBERTO; CAMPANA-FILHO, SERGIO PAULO. Insight into morphological, physicochemical and spectroscopic properties of beta-chitin nanocrystalline structures. Carbohydrate Polymers, v. 273, DEC 1 2021. (19/13656-8, 17/20973-4, 16/20970-2)

BORBA, KARLA R.; OLDONI, FERNANDA C. A.; MONARETTO, TATIANA; COLNAGO, LUIZ A.; FERREIRA, MARCOS D.. Selection of industrial tomatoes using TD-NMR data and computational classification methods. Microchemical Journal, v. 164, MAY 2021. (20/07017-0, 19/13656-8)

OLDONI, FERNANDA C. A.; BERNARDO, MARCELA P.; FILHO, JOSEMAR G. OLIVEIRA; DE AGUIAR, ALINE C.; MOREIRA, FRANCYS K. V.; MATTOSO, LUIZ H. C.; COLNAGO, LUIZ A.; FERREIRA, MARCOS D.. Valorization of mangoes with internal breakdown through the production of edible films by continuous solution casting. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 145, JUN 2021. (18/07860-9, 19/13656-8, 18/24612-9)

BIZZANI, MARILIA; FLORES, DOUGLAS WILLIAM MENEZES; COLNAGO, LUIZ ALBERTO; FERREIRA, MARCOS DAVID. Monitoring of soluble pectin content in orange juice by means of MIR and TD-NMR spectroscopy combined with machine learning. Food Chemistry, v. 332, DEC 1 2020. (19/13656-8, 13/23479-0)

SILVA, DANIELLA SOUZA; FACCHINATTO, WILLIAM MARCONDES; SANTOS, DANILO MARTINS DOS; BONI, FERNANDA ISADORA; VALDES, TALITA ALVARENGA; LEITAO, ANDREI; GREMIAO, MARIA PALMIRA DAFLON; COLNAGO, LUIZ ALBERTO; CAMPANA-FILHO, SERGIO PAULO; RIBEIRO, SIDNEY JOSE LIMA. N-(2-hydroxy)-propyl-3-trimethylammonium, O-palmitoyl chitosan: Synthesis, physicochemical and biological properties. International Journal of Biological Macromolecules, v. 178, p. 558-568, MAY 1 2021. (16/20970-2, 17/20973-4, 19/13656-8)

BIZZANI, MARILIA; MENEZES FLORES, DOUGLAS WILLIAM; MORAES, TIAGO BUENO; COLNAGO, LUIZ ALBERTO; FERREIRA, MARCOS DAVID; FILLET SPOTO, MARTA HELENA. Non-invasive detection of internal flesh breakdown in intact Palmer mangoes using time-domain nuclear magnetic resonance relaxometry. Microchemical Journal, v. 158, NOV 2020. (13/23479-0, 14/22126-9, 19/13656-8)

SPRICIGO, POLIANA CRISTINA; BARBOSA CORREIA, BANNY SILVA; BORBA, KARLA RODRIGUES; TAVER, ISABELA BARROSO; MACHADO, GUILHERME DE OLIVEIRA; WILHELMS, RENAN ZIEMANN; KENG QUEIROZ JUNIOR, LUIZ HENRIQUE; JACOMINO, ANGELO PEDRO; COLNAGO, LUIZ ALBERTO. lassical Food Quality Attributes and the Metabolic Profile of Cambuci, a Native Brazilian Atlantic Rainforest Frui. Molecules, v. 26, n. 12, JUN 2021. (14/12606-3, 18/00927-0, 19/05015-2, 19/13656-8)

MARINS OCAMPOS, FERNANDA MARIA; BORIM DE SOUZA, ANA JULIA; ANTAR, GUILHERME MEDEIROS; WOUTERS, FELIPE CHRISTOFF; COLNAGO, LUIZ ALBERTO. Phytotoxicity of Schiekia timida Seed Extracts, a Mixture of Phenylphenalenones. Molecules, v. 26, n. 14, JUL 2021. (14/50918-7, 12/25299-6, 19/13656-8, 18/00504-2)

FACCHINATTO, WILLIAM MARCONDES; GALANTE, JOANA; MESQUITA, LETICIA; SILVA, DANIELLA SOUZA; DOS SANTOS, DANILO MARTINS; MORAES, TIAGO BUENO; CAMPANA-FILHO, SERGIO PAULO; COLNAGO, LUIZ ALBERTO; SARMENTO, BRUNO; DAS NEVES, JOSE. Clotrimazole-loaded N -(2-hydroxy)-propyl-3-trimethylammonium, O -palmitoyl chitosan nanoparticles for topical treatment of vulvovaginal candidiasis. Acta Biomaterialia, v. 125, p. 312-321, APR 15 2021. (16/20970-2, 17/20973-4, 19/13656-8, 19/11135-0)

CONSOLO, NARA R. B.; SILVA, JULIANA; BUARQUE, VICENTE M.; BARBOSA, LUIS C.; PADILLA, ANGEL H.; COLNAGO, LUIZ A.; NETTO, ARLINDO SARAN; GERRARD, DAVID E.; SILVA, SAULO L.. Metabolomic signature of genetic potential for muscularity in beef cattle. ANIMAL BIOTECHNOLOGY, v. 33, n. 6, p. 10-pg., 2021-05-25. (16/17433-5, 16/01320-7, 19/13656-8)

COLNAGO, LUIZ ALBERTO; TREVISOL, IARA MARIA; VOSS RECH, DAIANE; FORATO, LUCIMARA APARECIDA; IGREJA DO NASCIMENTO MITRE, CIRLEI; GAGLIARDI LEITE, JOSE PAULO; GIGLIOTI, RODRIGO; OKINO, CINTIA HIROMI. Simple, Low-Cost and Long-Lasting Film for Virus Inactivation Using Avian Coronavirus Model as Challenge. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, v. 17, n. 18, p. 9-pg., 2020-09-01. (19/13656-8)