ENZYTERP: enzymatic biotransformation of terpenes for the production of bioaromas

• Grant number: 20/06814-3
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2020
• End date: May 31, 2023
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Juliano Lemos Bicas
• Grantee: Juliano Lemos Bicas
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Glaucia Maria Pastore ; Julian Martínez ; Marcus Bruno Soares Forte ; Ruann Janser Soares de Castro

Abstract

The global flavor market is estimated to reach US$ 26.3 billion in 2020, with an increasing demand for healthier and natural products. In this sense, the generation of aromas via biotransformation becomes more attractive, since it occurs under mild conditions, do not generate toxic wastes and can yield products with potentially competitive prices. Brazil presents an ideal conjuncture to provide bioaromas from the biotransformation of terpenes, as it has abundant and low-priced terpene substrates, besides its international prominence in the scientific development in this area. Thus, the present project aims to study bioflavor production processes involving the biotransformation of terpenes by isolated enzymes, instead of microbial cells, aiming to increase productivity by reducing the limitations associated with mass transfer. The proposed project focuses on the biotransformation of R-(+)-limonene into R-(+)-±-terpineol by the enzyme ±-terpineol deidratase of Sphingobium sp., for which the technical feasibility of using supercritical CO2 as a reaction medium will be examined in order to addition to increase yields and to optimize the recovery and purification of R-(+)-±-terpineol. Thus, it is expected that this project can meet industrial demands for more effective, cost-effective and robust biotechnological solutions to produce bioaromas. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAULINO, BRUNO N.; SILVA, GLORIA N. S.; ARAUJO, FABIO F.; NERI-NUMA, IRAMAIA A.; PASTORE, GLAUCIA M.; BICAS, JULIANO L.; MOLINA, GUSTAVO. Beyond natural aromas: The bioactive and technological potential of monoterpenes. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 128, p. 14-pg., 2022-08-26. (16/21619-7, 20/06814-3, 20/15163-6)

FELIPE, LORENA DE OLIVEIRA; BICAS, JULIANO LEMOS; BOUHOUTE, MERYEM; VODO, SEKOVE; TAARJI, NOAMANE; NAKAJIMA, MITSUTOSHI; NEVES, MARCOS A.. Formulation and physicochemical stability of oil-in-water nanoemulsion loaded with a-terpineol as flavor oil using Quillaja saponins as natural emulsifier. Food Research International, v. 153, p. 10-pg., 2022-03-01. (20/06814-3)

FELIPE, LORENA DE OLIVEIRA; BICAS, JULIANO LEMOS; MOREIRA, RAFAEL CHELALA; ALEXANDRINO, THAIS DOLFINI; CHANGWATCHAI, TEETACH; NAKAJIMA, MITSUTOSHI; NEVES, MARCOS A.. Elaboration and Properties of an Oil-in-Water Nanoemulsion Loaded with a Terpene-Enriched Oil Mixture Obtained Biotechnologically. ACS AGRICULTURAL SCIENCE & TECHNOLOGY, v. 1, n. 6, p. 9-pg., 2021-12-20. (20/06814-3, 16/21619-7)

FELIPE, LORENA DE OLIVEIRA; BICAS, JULIANO LEMOS; BOUHOUTE, MERYEM; NAKAJIMA, MITSUTOSHI; NEVES, MARCOS A.. omprehensive study of alpha-terpineol-loaded oil-in-water (O/W) nanoemulsion: interfacial property, formulation, physical and chemical stabilit. NPJ SCIENCE OF FOOD, v. 5, n. 1, NOV 15 2021. (20/06814-3)

MOREIRA, RAFAEL CHELALA; LEONARDI, GISLAINE RICCI; BICAS, JULIANO LEMOS. Lipase-mediated alcoholysis for in situ production of ester bioaromas in licuri oil for cosmetic applications. Journal of Biotechnology, v. 392, p. 9-pg., 2024-09-10. (20/06814-3)

GUSTAVO APARECIDO MARTINS; JULIANO LEMOS BICAS. Antifungal activity of essential oils of tea tree, oregano, thyme, and cinnamon, and their components. Brazilian Journal of Food Technology, v. 27, 2024-02-26. (20/06814-3)

MOREIRA, RAFAEL CHELALA; DE MELO, RAYANNE PRISCILLA FRANCA; MARTINEZ, JULIAN; MAROSTICA JUNIOR, MARIO ROBERTO; PASTORE, GLAUCIA MARIA; ZORN, HOLGER; BICAS, JULIANO LEMOS. Supercritical CO2 as a Valuable Tool for Aroma Technology. Journal of Agricultural and Food Chemistry, v. 71, n. 24, p. 12-pg., 2023-06-08. (21/01008-1, 15/13320-9, 20/06814-3, 17/11245-5, 15/50333-1, 19/13465-8, 18/11069-5)

GASPARETTO, BRUNA DOS REIS; MOREIRA, RAFAEL CHELALA; DE MELO, RAYANNE PRISCILLA FRANCA; LOPES, ALINE DE SOUZA; ROCHA, LILIANA DE OLIVEIRA; PASTORE, GLAUCIA MARIA; BICAS, JULIANO LEMOS; MARTINEZ, JULIAN; STEEL, CAROLINE JOY. Effect of supercritical CO2 fractionation of Tahiti lemon (Citrus latifolia Tanaka) essential oil on its antifungal activity against predominant molds from pan bread. Food Research International, v. 162, p. 11-pg., 2022-12-01. (20/06814-3)

FELIPE, LORENA DE OLIVEIRA; BICAS, JULIANO LEMOS; CHANGWATCHAI, TEETACH; ABAH, EMMANUEL OWOICHO; NAKAJIMA, MITSUTOSHI; NEVES, MARCOS A.. Physical stability of α-terpineol-based nanoemulsions assessed by direct and accelerated tests using photo centrifuge analysis. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 205, p. 10-pg., 2024-07-24. (20/06814-3)

ALEXANDRINO, THAIS DOLFINI; DE MEDEIROS, TIAGO DANIEL MADUREIRA; RUIZ, ANA LUCIA TASCA GOIS; FAVARO, DENIZE CRISTINA; PASTORE, GLAUCIA MARIA; BICAS, JULIANO LEMOS. Structural properties and evaluation of the antiproliferative activity of limonene-1,2-diol obtained by the fungal biotransformation of R-(+)- and S-(-)-limonene. CHIRALITY, v. 34, n. 6, p. 7-pg., 2022-04-14. (20/06814-3, 16/21619-7)