Xylooligosaccharides production from agroindustrial wastes and technological application in meat and plant-based products

• Grant number: 22/15221-1
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2023
• End date: January 31, 2026
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Rosana Goldbeck Coelho
• Grantee: Rosana Goldbeck Coelho
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Ana Carla Kawazoe Sato ; Marise Aparecida Rodrigues Pollonio ; Patricia Felix Avila ; Rosiane Lopes da Cunha
• Associated scholarship(s): 25/15158-6 - Brewer's Spent Grain utilization for the production of xylooligosaccharides and bioadsorbent agents, BP.IC; 24/19336-3 - Production of Xylo-oligosaccharides from Lignocellulosic Biomass for Use in Hybrid Meat Systems and Meat Analog Formulations, BP.IC

Abstract

Xylo-oligosaccharides (XOS) are non-digestible oligomers originating from the hemicellulose present in lignocellulosic materials, which show great potential in functional and technological applications in the food industry. As a food ingredient, XOS have favorable technological characteristics, including stability in acidic media, heat resistance, water retention capacity, being suitable for applications as thickening agents and emulsion stabilizers and gel formulations. The consumption of such compounds also contributes to several beneficial health effects due to their prebiotic properties. In this sense, this project aims to produce XOS via enzymatic hydrolysis of xylan extracted from sugarcane bagasse, as well as how to evaluate the effects of its incorporation in the stabilization of meat emulsions (traditional and hybrid) and meat analogs "plant-based" products. Initially, xylan will be extracted from sugarcane bagasse in order to be used as a substrate in the production of XOS, using an enzymatic cocktail rich in endoxylanase (NS50030-Novozymes®). To evaluate the effects of the incorporation of XOS in meat emulsions and plant-based products, combinations of XOS and other hydrocolloids, such as carrageenan and starch of cassava, will be carried out in order to select the ideal proportions. Finally, the technological and rheological properties will be evaluated, as well as the potential of these ingredients in the elaboration of meat (traditional and hybrid) and plant-based products, within the quality standards and, at the same time, with functional appeal, from the perspective of the sustainable use of agro-industrial residues. Many works report the prebiotic properties of XOS and its use as functional ingredients, however little is investigated regarding its structuring properties, which characterizes the project as original and innovative, subject to the development of patentable technology of great relevance to the food industry. It is also important to mention that this proposal is closely linked to the completed FAPESP regular grant project (2019/08542-3) "Enzyme biotechnology for the production of xylo-oligosaccharides from lignocellulosic agro-industrial residues in order to obtain symbiotics" which provided relevant results for the production of XOS from sugarcane biomass, proving its prebiotic/symbiotic properties, and in this way it will help us in the execution of this proposal. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARROSO, TIAGO LINHARES CRUZ TABOSA; CASTRO, LUIZ EDUARDO NOCHI; DA CRUZ, ELDER PACHECO; FERREIRA, VANESSA COSME; DA ROSA, RAFAEL GABRIEL; GOLDBECK, ROSANA; FORSTER-CARNEIRO, TANIA. Subcritical water hydrolysis followed by pre-purification of cashew apple bagasse hydrolysates to produce fermentable sugar. Food Research International, v. 219, p. 13-pg., 2025-11-01. (18/14938-4, 21/04096-9, 22/15221-1, 23/02064-8, 24/06628-6)

DA SILVA, MARCOS F.; FLAIBAM, BARBARA; DE MELO, ALLAN H. F.; SAMPAIO, ULLIANA; CLERICI, MARIA TERESA P. S.; GOLDBECK, ROSANA. Optimization of enzymatic hydrolysis of cellulose extracted from bamboo culm for bioethanol production by Saccharomyces cerevisiae modified via CRISPR/Cas9. Food Research International, v. 192, p. 9-pg., 2024-07-19. (22/15221-1, 21/09527-8)

RODRIGUES, DANIELLE MATIAS; DA SILVA, MARCOS FELLIPE; DE MELO, ALLAN HENRIQUE FELIX; CARVALHO, PRISCILA HOFFMANN; BAUDEL, HENRIQUE MACEDO; GOLDBECK, ROSANA. Sustainable synthesis pathways: Bacterial nanocellulose from lignocellulosic biomass for circular economy initiatives. Food Research International, v. 192, p. 9-pg., 2024-08-01. (22/15221-1)

MARTINS, MANOELA; GOLDBECK, ROSANA. Integrated biorefinery for xylooligosaccharides, pectin, and bioenergy production from orange waste. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, v. 17, n. 6, p. 14-pg., 2023-10-14. (22/15221-1, 19/08542-3, 20/02871-2)

DA SILVA, MARCOS FELLIPE; DE MELO, ALLAN HENRIQUE FELIX; MIRANDA, YURI RAMATIS SILVA; AVILA, PATRICIA FELIX; SAMPAIO, ULLIANA; SCHMIDT, FLAVIO LUIS; CLERICI, MARIA TERESA PEDROSA SILVA; GOLDBECK, ROSANA. Enzymatic depolymerization of arabinoxylan from bamboo culm for high and fast production of high purity short chain xylo-oligosaccharides. Cellulose, v. 31, n. 5, p. 19-pg., 2024-02-20. (22/15221-1, 21/09527-8)

PASCHOA, JOAO L. F.; AVILA, PATRICIA FELIX; SILVA, MARCOS F.; DE MELO, ALLAN HENRIQUE F.; DA CUNHA, ROSIANE LOPES; GOLDBECK, ROSANA. Development of carrageenan-based emulsion gel as a vehicle to transport xylo-oligosaccharides and its functional properties. FOOD & FUNCTION, v. 16, n. 6, p. 15-pg., 2025-02-14. (22/15221-1)

SOARES, MARCELO GOMES; OKURO, PAULA KIYOMI; DA SILVA, MARCOS FELLIPE; GOLDBECK, ROSANA; CUNHA, ROSIANE LOPES. Ultra-Long-Chain Sorbitol Esters Tailoring Thermo-Responsive Rheological Properties of Oleogels. FOODS, v. 14, n. 6, p. 19-pg., 2025-03-18. (19/27354-3, 22/05719-2, 22/15221-1, 18/20308-3, 21/09527-8)

BARROSO, TIAGO LINHARES CRUZ TABOSA; CASTRO, LUIZ EDUARDO NOCHI; DA ROSA, RAFAEL GABRIEL; BRACKMANN, RODRIGO; GOLDBECK, ROSANA; FORSTER-CARNEIRO, TANIA. Obtaining value-added products from cashew apple bagasse: A sustainable alternative achieved through a hydrothermal process. Food Research International, v. 208, p. 15-pg., 2025-05-01. (18/14938-4, 22/15221-1, 21/04096-9, 23/02064-8)

FLAIBAM, BARBARA; MEIRA, CASSIO SANTANA; NERY, TATIANA BARRETO ROCHA; GALLAND, FABIANA; PACHECO, MARIA TERESA BERTOLDO; GOLDBECK, ROSANA. Low-cost protein extracts and hydrolysates from plant-based agro-industrial waste: Inputs of interest for cultured meat. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 93, p. 14-pg., 2024-04-01. (22/15221-1)

DA SILVA, MARCOS F.; BUENO, DANILO; GOLDBECK, ROSANA. Challenges and trends in the industrial production and application of cellooligosaccharides. CURRENT OPINION IN FOOD SCIENCE, v. 63, p. 9-pg., 2025-04-18. (22/15221-1, 21/09527-8)

DA SILVA, MARCOS FELLIPE; SGANZERLA, WILLIAM GUSTAVO; AVILA, PATRICIA FELIX; CARNEIRO, TANIA FORSTER; CLERICI, MARIA TERESA PEDROSA SILVA; GOLDBECK, ROSANA. Evaluation of the functional properties of short-chain xylooligosaccharides obtained from the enzymatic hydrolysis of bamboo culm. BIOACTIVE CARBOHYDRATES AND DIETARY FIBRE, v. 32, p. 7-pg., 2024-06-29. (22/15221-1, 21/09527-8, 18/14938-4, 19/26925-7)

PASCHOA, JOAO L. F.; AVILA, PATRICIA F.; DA COSTA, GILMAR F.; RIBEIRO, ANA PAULA B.; GRIMALDI, RENATO; DA CUNHA, ROSIANE L.; POLLONIO, MARISE A. R.; GOLDBECK, ROSANA. Application of Xylo-Oligosaccharide-Rich Gel Emulsion as a Fat Replacer in Sausages. FOODS, v. 13, n. 22, p. 14-pg., 2024-11-01. (22/15221-1)

FLAIBAM, BARBARA; DA SILVA, MARCOS F.; DE MELO, ALLAN H. FELIX; CARVALHO, PRISCILA HOFFMANN; GALLAND, FABIANA; PACHECO, MARIA TERESA BERTOLDO; GOLDBECK, ROSANA. Non-animal protein hydrolysates from agro-industrial wastes: A prospect of alternative inputs for cultured meat. Food Chemistry, v. 443, p. 13-pg., 2024-01-26. (22/15221-1)

BARROSO, TIAGO LINHARES CRUZ TABOSA; FERREIRA, VANESSA COSME; CASTRO, LUIZ EDUARDO NOCHI; DA ROSA, RAFAEL GABRIEL; DE SOUZA, LUIZ GUSTAVO ANTONIO; GOLDBECK, ROSANA; FORSTER-CARNEIRO, TANIA. Bioenergy and bioproducts from cashew apple bagasse: a scientometric overview and strategies for sustainable utilization. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, v. N/A, p. 30-pg., 2024-12-17. (21/04096-9, 24/06628-6, 23/02064-8, 18/14938-4, 22/15221-1)

RODRIGUES, DANIELLE MATIAS; SILVA, MARCOS FELLIPE DA; ALMEIDA, FRANCISCO LUCAS CHAVES; FORTE, MARCUS BRUNO SOARES; MARTIN, CARLOS; BARUD, HERNANE DA SILVA; BAUDEL, HENRIQUE MACEDO; GOLDBECK, ROSANA. A green approach to biomass residue valorization: Bacterial nanocellulose production from agro-industrial waste. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 56, p. 17-pg., 2024-02-10. (22/15221-1, 19/03399-8)

Filed patent(s) as a result of this research project