Multimodal investigation of epileptogenesis with emphasis in the implementation of new animal models and new tools

• Grant number: 11/50680-2
• Support Opportunities: Research Projects - Thematic Grants
• Start date: April 01, 2012
• End date: March 31, 2016
• Field of knowledge: Health Sciences - Medicine
• Agreement: CNPq - Pronex
• Principal Investigator: Iscia Teresinha Lopes Cendes
• Grantee: Iscia Teresinha Lopes Cendes
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Pesquisadores principais:( Últimos ): Debora Amado Scerni ; Maria da Graca Naffah Mazzacoratti
• Pesquisadores principais:( Antigos ): Fernando Cendes
• Associated scholarship(s): 14/11277-6 - Structural and biochemical evaluation by magnetic resonance imaging in temporal lobe epilepsy model in rats with and without status epilepticus, BP.DR; 13/08635-5 - TRANSCRIPTOME ANALYSIS IN ANIMAL MODELS OF MESIAL TEMPORAL LOBE EPILEPSY, BP.DR; 13/24801-2 - Analysis and Development of Protocols in Bioinformatics for Epigenetic Studies, BP.MS; + associated scholarships 13/09214-3 - Differencially expressed proteins indentification in differents tissues from epilepsy experimental models, BP.MS; 12/22635-5 - Resistance to pilocarpine-induced status epilepticus: assessment of hormonal mechanisms involved in epileptogenesis, BP.PD; 12/21548-1 - Multimodal investigation of epileptogenesis with emphasis in the implementation of new animal models and new tools, BP.PD; 12/10764-5 - Evaluation of cellular effects of deep brain stimulation of the anterior nucleus of the thalamus in experimental models of temporal lobe epilepsy, BP.DD; 12/10702-0 - Multimodal investigation of epileptogenesis using new models and new tools, BE.PQ; 08/00068-6 - Proteomic evaluation of temporal lobe epilepsy in human and rat sujected to pilocarpine model, receiving or not neuroprotector agent R-PIA pretreatment., BP.PD - associated scholarships

Abstract

Epilepsy affects about 1% to 2% of world population, and the mesial temporal lobe epilepsy (TLE) the most frequent, representing 40% of patients, these are often refractory to medicaI treatment. One of the most used tools to try to understand the pathophysiology of MTLE is induced animal models. Despite several animal models currently available and widely studied, most of these lesions present in the central nervous system and phenotypic events that are not commonly identified in patients with TLE, which is primarily neuronal loss in hippocampal regions and several initial insults such as: ischemia, trauma, and status epilepticus (SE). That said, I propose to work with animal models recently described (Norwood et aI., 2010 and Navarro-Mora G, 2009) whose main advantage to generate a discrete lesion, actually closer to that found in patients with TLE and without the initial insult precipitating the type SE. With these models we propose to use a series of tools to investigate various phenomena and biological pathways that intersect in the determination of epileptogenesis in TLE. This strategy will provide an interdisciplinary approach that goes from structural neuroimaging to molecular biology, through neurophysiology, neurochemistry and bio-informatics. Moreover, as all groups participating in the proposal have extensive experience in research in epilepsy, the results obtained here can be compared with those previously produced in other animal models and those observed in series of patients. We believe our strategy of integration of knowledge proposed here will generate new knowledge, comprehensive and integrated on epileptogenesis in mesial temporal lobe structures and have a chance to make a big impact in the field of applied neuroscience. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ALMEIDA, ALEXANDRE APARECIDO; DA SILVA, SERGIO GOMES; LOPIM, GLAUBER MENEZES; CAMPOS, DIEGO VANNUCCI; FERNANDES, JANSEN; CABRAL, FRANCISCO ROMERO; ARIDA, RICARDO MARIO. Physical exercise alters the activation of downstream proteins related to BDNF-TrkB signaling in male Wistar rats with epilepsy. Journal of Neuroscience Research, v. 96, n. 5, p. 911-920, MAY 2018. (13/12692-4, 14/00035-1, 13/13725-3, 11/50680-2)

DE ALMEIDA, ALEXANDRE APARECIDO; DA SILVA, SERGIO GOMES; LOPIM, GLAUBER MENEZES; CAMPOS, DIEGO VANNUCCI; FERNANDES, JANSEN; CABRAL, FRANCISCO ROMERO; ARIDA, RICARDO MARIO. Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy. Neurochemical Research, v. 42, n. 4, p. 1230-1239, APR 2017. (13/12692-4, 11/50680-2, 14/00035-1)

COVOLAN, LUCIENE; DE ALMEIDA, ANTONIO-CARLOS G.; AMORIM, BEATRIZ; CAVARSAN, CLARISSA; MIRANDA, MAISA FERREIRA; AARAO, MAYRA C.; MADUREIRA, ANA PAULA; RODRIGUES, ANTONIO M.; NOBREGA, JOSE N.; MELLO, LUIZ E.; et al. Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats. PLoS One, v. 9, n. 6, JUN 3 2014. (12/50950-2, 11/50680-2)

SCHOR, B.; GOMES DA SILVA, S.; DE ALMEIDA, A. A.; PEREIRA, C. A. B.; ARIDA, R. M.. Plasma brain-derived neurotrophic factor is higher after combat training (Randori) than incremental ramp test in elite judo athletes. Brazilian Journal of Medical and Biological Research, v. 52, n. 4, 2019. (13/12692-4, 11/50680-2)

FERNANDES, JANSEN; VIEIRA, ANDRE SCHWAMBACH; KRAMER-SOARES, JULIANA CARLOTA; DA SILVA, EDUARDO ALVES; LEE, KIL SUN; LOPES-CENDES, ISCIA; ARIDA, RICARDO MARIO. Hippocampal microRNA-mRNA regulatory network is affected by physical exercise. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1862, n. 8, p. 1711-1720, AUG 2018. (13/13725-3, 11/50680-2)

AMORIM, BEATRIZ O.; COVOLAN, LUCIENE; FERREIRA, ELENN; BRITO, JOSE GERALDO; NUNES, DIEGO P.; DE MORAIS, DAVID G.; NOBREGA, JOSE N.; RODRIGUES, ANTONIO M.; DEALMEIDA, ANTONIO CARLOS G.; HAMANI, CLEMENT. Deep brain stimulation induces antiapoptotic and anti-inflammatory effects in epileptic rats. JOURNAL OF NEUROINFLAMMATION, v. 12, SEP 4 2015. (12/50950-2, 12/10764-5, 11/50680-2)

RAMOS, FABIANE OCHAI; RODRIGUES CARREIRO, LUIZ RENATO; SCORZA, FULVIO ALEXANDRE; CYSNEIROS, ROBERTA MONTERAZZO. Prejuízo das funções executivas no modelo experimental de epilepsia do lobo temporal. Arquivos de Neuro-Psiquiatria, v. 74, n. 6, p. 470-477, JUN 2016. (11/50680-2)

AMORIM, BEATRIZ OLIVEIRA; HAMANI, CLEMENT; FERREIRA, ELENN; MIRANDA, MAISA FERREIRA; FERNANDES, MARIA JOSE S.; RODRIGUES, ANTONIO M.; DE ALMEIDA, ANTONIO-CARLOS G.; COVOLAN, LUCIENE. Effects of A1 receptor agonist/antagonist on spontaneous seizures in pilocarpine-induced epileptic rats. Epilepsy & Behavior, v. 61, p. 168-173, AUG 2016. (12/50950-2, 12/10764-5, 11/50680-2)