Understanding the emulsion behaviour of fermentation mixtures in the microbial production of diesel and jet-like biofuels

• Grant number: 11/51707-1
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Duration: July 01, 2012 - June 30, 2014
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Convênio/Acordo: BE-BASIC Consortium
• Principal Investigator: Rosiane Lopes da Cunha
• Grantee: Rosiane Lopes da Cunha
• Principal researcher abroad: Maria Cuellar Soares
• Institution abroad: Delft University of Technology (TU Delft), Netherlands
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 13/02204-2 - Understanding the emulsion behaviour of fermentation mixtures in the microbial production of diesel and jet-like biofuels, BP.TT; 12/17931-4 - Understanding the emulsion behaviour of fermentation mixtures in the microbial production of diesel and jet-like biofuels, BP.TT

Abstract

Within the many possible renewable energy resources, biofuels dominate the current market. However, given the tight economic margins of the (bio)fuels sector, it is the design of overall cost-efficient processes what will determine the large scale implementation of these processes. Compared to alcohol biofuels (e.g. ethanol), the extracellular production of diesel and jet-like biofuels offers advantages in terms of product separation from the fermentation broth. However the microbial production of diesel and jetfuel-like biofuels results in a four-phase (S-L-L-G) mixture in the reactor (microbial cells, aqueous medium, lipid product and fermentation gas), which shows emulsion characteristics. Although there have been studies on the emulsion behavior of those mixtures, little emphasis has been given to a systematic study of factors relevant for large scale separation operation. Understanding the mechanisms behind emulsion formation is key for a rationale feedstock (and the pre-treatment associated to it) selection, for defining process (fermentation and product recovery) conditions and for identifying targets for synthetic biology of producing micro-organisms (e.g. robustness at low pH, high salt concentration). Thus, this proposal aims at cooperation between two research groups that can contribute to the consolidation of processes for production of biofuels. The group of Delft University is expert in bio-processes, while the group at the University of Campinas has experience in emulsions and protein-based emulsifiers. In particular, it is expected to understand the mechanisms that lead to the formation of emulsion in the fermentation process to develop a separation process efficient and inexpensive, but at the same time preserving the microbial cell viability. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIANO, ALBERTO CLAUDIO; SALDANA, ERICK; CAMPESTRINI, LUCIANO HENRIQUE; CHIORATO, ALISSON FERNANDO; DUARTE AUGUSTO, PEDRO ESTEVES. Correlating the properties of different carioca bean cultivars (Phaseolus vulgaris) with their hydration kinetics. Food Research International, v. 107, p. 182-194, MAY 2018. (14/16998-3, 16/18052-5, 11/51707-1)

SANTOS, TATIANA PORTO; CUNHA, ROSIANE LOPES. Role of process variables on the formation and in vitro digestion of gellan gels. Carbohydrate Polymers, v. 192, p. 111-117, JUL 15 2018. (15/21677-4, 11/51707-1)

HEERES, ARJAN S.; PICONE, CAROLINA S. F.; VAN DER WIELEN, LUUK A. M.; CUNHA, ROSIANE L.; CUELLAR, MARIA C.. Microbial advanced biofuels production: overcoming emulsification challenges for large-scale operation. TRENDS IN BIOTECHNOLOGY, v. 32, n. 4, p. 221-229, APR 2014. (11/51707-1, 12/14003-9)

VIEIRA, J. M.; MANTOVANI, R. A.; RAPOSO, M. F. J.; COIMBRA, M. A.; VICENTE, A. A.; CUNHA, R. L.. Effect of extraction temperature on rheological behavior and antioxidant capacity of flaxseed gum. Carbohydrate Polymers, v. 213, p. 217-227, JUN 1 2019. (09/54137-1, 16/05448-8, 06/03263-9, 11/51707-1)

MARSON, GABRIELA VOLLET; VITOR PEREIRA, DEBORA TAMIRES; DA COSTA MACHADO, MARIANA TEIXEIRA; DI LUCCIO, MARCO; MARTINEZ, JULIAN; BELLEVILLE, MARIE-PIERRE; HUBINGER, MIRIAM DUPAS. Ultrafiltration performance of spent brewer's yeast protein hydrolysate: Impact of pH and membrane material on fouling. Journal of Food Engineering, v. 302, AUG 2021. (18/15737-2, 17/23670-2, 16/18465-8, 11/51707-1, 18/04067-6, 09/54137-1, 11/06083-0)

MARSON, GABRIELA VOLLET; DA COSTA MACHADO, MARIANA TEIXEIRA; SOARES DE CASTRO, RUANN JANSER; HUBINGER, MIRIAM DUPAS. Sequential hydrolysis of spent brewer's yeast improved its physico-chemical characteristics and antioxidant properties: A strategy to transform waste into added-value biomolecules. Process Biochemistry, v. 84, p. 91-102, SEP 2019. (18/04067-6, 11/51707-1, 16/18465-8)

MARSON, GABRIELA VOLLET; SOARES DE CASTRO, RUANN JANSER; DA COSTA MACHADO, MARIANA TEIXEIRA; ZANDONADI, FLAVIA DA SILVA; DE FREITAS QUEIROZ BARROS, HELENA DIAS; MAROSTICA JUNIOR, MARIO ROBERTO; SUSSULINI, ALESSANDRA; HUBINGER, MIRIAM DUPAS. Proteolytic enzymes positively modulated the physicochemical and antioxidant properties of spent yeast protein hydrolysates. Process Biochemistry, v. 91, p. 34-45, APR 2020. (06/03263-9, 11/51707-1, 09/54137-1, 16/18465-8, 15/50333-1, 18/04067-6, 17/04231-8, 15/24351-2, 11/06083-0)

DIAS MEIRELLES, AURELIANO AGOSTINHO; DA CUNHA, ROSIANE LOPES; GOMBERT, ANDREAS KAROLY. The role of Saccharomyces cerevisiae in stabilizing emulsions of hexadecane in aqueous media. Applied Microbiology and Biotechnology, v. 102, n. 7, p. 3411-3424, APR 2018. (11/51707-1)

VIEIRA, J. M.; ANDRADE, C. C. P.; SANTOS, T. P.; OKURO, P. K.; GARCIA, S. T.; RODRIGUES, I, M.; VICENTE, A. A.; CUNHA, R. L.. Flaxseed gum-biopolymers interactions driving rheological behaviour of oropharyngeal dysphagia-oriented products. FOOD HYDROCOLLOIDS, v. 111, FEB 2021. (06/03263-9, 17/18109-0, 11/51707-1, 09/54137-1, 16/05448-8, 18/20308-3)

DA SILVA CARVALHO, ANA GABRIELA; DA COSTA MACHADO, MARIANA TEIXEIRA; DE FREITAS QUEIROZ BARROS, HELENA DIAS; BETIM CAZARIN, CINTHIA BAU; MAROSTICA JUNIOR, MARIO ROBERTO; HUBINGER, MIRIAM DUPAS. Anthocyanins from jussara (Euterpe edulis Martius) extract carried by calcium alginate beads pre-prepared using ionic gelation. Powder Technology, v. 345, p. 283-291, MAR 1 2019. (11/51707-1, 09/54137-1, 11/06083-0)

VIEIRA, J. M.; OLIVEIRA JR, F. D.; SALVARO, D. B.; MAFFEZZOLLI, G. P.; DE MELLO, J. D. B.; VICENTE, A. A.; CUNHA, R. L.. Rheology and soft tribology of thickened dispersions aiming the development of oropharyngeal dysphagia-oriented products. CURRENT RESEARCH IN FOOD SCIENCE, v. 3, p. 19-29, NOV 2020. (06/03263-9, 11/51707-1, 09/54137-1, 16/05448-8)

MARSON, GABRIELA VOLLET; SATURNO, RAFAELA POLESSI; COMUNIAN, TALITA ALINE; CONSOLI, LARISSA; DA COSTA MACHADO, MARIANA TEIXEIRA; HUBINGER, MIRIAM DUPAS. Maillard conjugates from spent brewer's yeast by-product as an innovative encapsulating material. Food Research International, v. 136, OCT 2020. (18/04067-6, 11/51707-1, 15/11984-7, 15/24351-2, 06/03263-9, 16/18465-8, 11/06083-0, 12/50411-4, 09/54137-1)