| Grant number: | 12/50191-4 |
| Support Opportunities: | Research Projects - Thematic Grants |
| Start date: | December 01, 2012 |
| End date: | November 30, 2018 |
| Field of knowledge: | Biological Sciences - Biochemistry - Enzymology |
| Principal Investigator: | Maria Aparecida Juliano |
| Grantee: | Maria Aparecida Juliano |
| Host Institution: | Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil |
| Associated research grant(s): | 18/13588-0 - Multi - user equipment approved in grant 2012/50191-4: mass spectrometer impact II - Bruker, AP.EMU |
| Associated scholarship(s): | 15/01829-4 - Human Tissue Kallikrein (KLK1) and human plasma kallikrein on metabolism of semaphorins and neurotrophins,
BP.PD 14/18877-9 - Proteolytic enzymes in skin physiology and pathophysiology, BP.PD 14/18451-1 - Leptospira spp. strains database implemention by MALDI-TOF-MS and activity analysis of their secreted proteases, BP.IC + associated scholarships - associated scholarships |
Abstract
Considerable progress in the study of proteases was based on the development of synthetic peptides, which resulted in the chromogenic and fluorescent substrates. Quenched fluorescent peptides based on the transfer of energy by resonance of a fluorescent to a quancher group was the more recent and significant progress in the field that we are involve since 1990's. The synthesis of these FRET (Fluorescence Resonance Energy Transfer) peptides in solid phase linked to flexible resins and in systems of parallel synthesis allowed us to study the substrate specificity of several proteases by systematic variations in all positions of peptides with up to 10 amino acids in length. The project presented here is based on the development of modern techniques of organic synthesis and protein biochemistry for study of proteases involved in physiological and pathological processes. The proposals of this project are: Synthesis and assays with proteases of FRET peptide libraries in solid phase and in solution. Synthesis of protease inhibitors using non-natural amino acids. Assays as protease inhibitors of tellurium organic compounds and nitroolefins, particularly the cysteine proteases. Systematic studies will be done with human kallikreins, Plamodium and Leishmania proteases, processing peptidases and oligopeptidases. Virus, fungi and bactéria proteases were also included. (AU)
| Articles published in Agência FAPESP Newsletter about the research grant: |
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MÉTODO DE IDENTIFICAÇÃO DE COMPOSTOS ANTIVIRAIS, COMPOSTOS ANTIVIRAIS, MÉTODO DE AVALIAÇÃO DA FUNCIONALIDADE DOS COMPOSTOS ANTIVIRAIS, USO DOS COMPOSTOS ANTIVIRAIS E COMPOSIÇÃO FARMACÊUTICA ANTIVIRAL BR1020150065612 - Universidade de São Paulo (USP) ; Universidade Federal de São Paulo (UNIFESP) . Antonia Tavares do Amaral ; Erika Piccirillo ; Maria Aparecida Juliano ; Marcia Yuri Kondo - March 2015, 24