Modulation of oocyte nuclear maturation and its effects on cumulus-oocyte complexes and bovine embryo production in vitro

• Grant number: 13/05083-1
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2013
• End date: November 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcelo Fábio Gouveia Nogueira
• Grantee: Marcelo Fábio Gouveia Nogueira
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
• Associated researchers: Ciro Moraes Barros ; Eduardo Montanari Razza ; Mariana Fernandes Machado

Abstract

In vitro maturation (IVM) restricts the use of hormones in vivo preventing excessive spending and handling of the animal, and the harmful iatrogenic effects of ovarian overstimulation. However, the IVM systems are not comparably efficient to physiological events that occur in vivo, mainly concerning the synchronization between the nuclear and cytoplasmic maturation of the oocyte. Removed from the follicular environment (in vitro), break up the gap junctions, resulting in low levels of cAMP in the oocyte allowing resumption of meiosis and consequent spontaneous asynchronous cytoplasmic maturation. Simulated physiological oocyte maturation (SPOM) provides a complex pharmacological manipulation of IVM. This system inhibits meiotic progression in two distinct phases (pre-maturation and maturation), while it stimulates oocyte maturation with high doses of FSH, achieving better results compared to conventional IVM. Another bias of investigation regard meiotic arrest comprises natriuretic peptide precursor type C (NPPC) and its receptor (NPR2) system. The interaction between these molecules in cumulus cells, indirectly contributes to the maintenance of cAMP levels, leading to meiotic arrest. Therefore, the study aims to assess the overall panel of bovine oocyte maturation in vitro. Specifically, the project aim to elucidate NPPC-NPR2 meiotic arrest system and its potential application in IVM routine; to compare SPOM and conventional IVM overall and in the oocytes and cumulus cells transcriptomics and embryo produced. Finally, the project will provide robust data for the development of systems that maximize in vitro production of bovine embryos and that will be substantially availed by the thematic project (GIFT) recently granted by FAPESP (2012/50533-2). (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOTIGELLI, RAMON CESAR; RAZZA, EDUARDO MONTANARI; PIOLTINE, ELISA MARIANO; FONTES, PATRICIA KUBO; LANCELLOTTI SCHWARZ, KATIA REGINA; VERDE LEAL, CLAUDIA LIMA; GOUVEIA NOGUEIRA, MARCELO FABIO. Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos. REPRODUCTIVE BIOLOGY, v. 18, n. 1, p. 66-75, MAR 2018. (12/23409-9, 15/20379-0, 13/05083-1, 12/50533-2, 13/07730-4, 12/00170-0)

RAZZA, EDUARDO M.; PEDERSEN, HANNE S.; STROEBECH, LOTTE; FONTES, PATRICIA K.; KADARMIDEEN, HAJA N.; CALLESEN, HENRIK; PIHL, MARIA; NOGUEIRA, MARCELO F. G.; HYTTEL, POUL. Simulated physiological oocyte maturation has side effects on bovine oocytes and embryos. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, v. 36, n. 3, p. 413-424, MAR 2019. (13/05083-1, 12/23409-9, 12/50533-2)

BOTIGELLI, RAMON CESAR; RAZZA, EDUARDO MONTANARI; PIOLTINE, ELISA MARIANO; GOUVEIA NOGUEIRA, MARCELO FABIO. New approaches regarding the in vitro maturation of oocytes: manipulating cyclic nucleotides and their partners in crime. JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA, v. 21, n. 1, p. 35-44, JAN-MAR 2017. (12/23409-9, 13/05083-1, 12/50533-2, 13/07730-4)

RAZZA, EDUARDO M.; SUDANO, MATEUS J.; FONTES, PATRICIA K.; FRANCHI, FERNANDA F.; BELAZ, KATIA ROBERTA A.; SANTOS, PRISCILA H.; CASTILHO, ANTHONY C. S.; ROCHA, DANIELE F. O.; EBERLIN, MARCOS N.; MACHADO, MARIANA F.; et al. Treatment with cyclic adenosine monophosphate modulators prior to in vitro maturation alters the lipid composition and transcript profile of bovine cumulus-oocyte complexes and blastocysts. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 30, n. 10, p. 1314-1328, SEP 2018. (13/05083-1, 12/23409-9, 12/50533-2, 12/10737-8)

ROCHA, JOSE CELSO; PASSALIA, FELIPE JOSE; MATOS, FELIPE DELESTRO; TAKAHASHI, MARIA BEATRIZ; CINICIATO, DIEGO DE SOUZA; MASERATI, MARC PETER; ALVES, MAYRA FERNANDA; DE ALMEIDA, TAMIE GUIBU; CARDOSO, BRUNA LOPES; BASSO, ANDREA CRISTINA; et al. A Method Based on Artificial Intelligence To Fully Automatize The Evaluation of Bovine Blastocyst Images. SCIENTIFIC REPORTS, v. 7, AUG 9 2017. (11/06179-7, 06/06491-2, 13/05083-1, 12/50533-2, 12/20110-2)

ROCHA, JOSE CELSO; PASSALIA, FELIPE JOSE; MATOS, FELIPE DELESTRO; TAKAHASHI, MARIA BEATRIZ; MASERATI, JR., MARC PETER; ALVES, MAYRA FERNANDA; DE ALMEIDA, TAMIE GUIBU; CARDOSO, BRUNA LOPES; BASSO, ANDREA CRISTINA; GOUVEIA NOGUEIRA, MARCELO FABIO. Automatized image processing of bovine blastocysts produced in vitro for quantitative variable determination. SCIENTIFIC DATA, v. 4, DEC 19 2017. (16/19004-4, 11/06179-7, 06/06491-2, 13/05083-1, 12/50533-2, 12/20110-2)

ROCHA, J. C.; MATOS, F. D.; BASSO, A. C.; HICKMAN, C. F. L.; LAVERY, S.; NOGUEIRA, M. F. G.. Using artificial intelligence to improve blastocyst morphology evaluation. Human Reproduction, v. 32, p. 2-pg., 2017-07-01. (12/50533-2, 06/06491-2, 13/05083-1, 12/20110-2)