Autophagy as a protective mechanism in senescent rats

• Grant number: 13/20073-2
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2014
• End date: August 31, 2016
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Soraya Soubhi Smaili
• Grantee: Soraya Soubhi Smaili
• Host Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Aging is associated with development of metabolic disorders and neurodegenerative diseases triggered by the reduction of antioxidant defenses, with alteration in apoptotic and autophagy processes. Studies reported autophagy as a strategy for neuroprotection in aging and neurodegenerative diseases, although little is known about its role in peripheral tissues. The scientific challenges of this project will determine the mode of action of lithium on the striatum tissue and liver in young and aged animals. Female Wistar rats will be divided in two groups: young (4-6 months) and aged (22-26 months). Young animals will be sorted in two subgroups differing in liquid solution: C group, control that will receive distilled water ; L group, receiving solution of lithium in neuroprotective concentration. The aged group will be sorted in two subgroups: S group, which receives distilled water; SL group, receiving solution of lithium in neuroprotective concentration. After 30 days of experiment will be analyzed the behavior test, autophagy, oxidative stress in liver and striatum, beyond lipid profile in serum of young and aged rats. It is expected an antioxidant or a cytoprotective activity of lithium, with a increase of autophagy. We can also analyze the effect of lithium in the prevention of oxidative stress and mitochondrial damage associated with aging. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARI LUMINOSA MULER; FERNANDA ANTUNES; GABRIEL CICOLIN GUARACHE; RAFAELA BRITO OLIVEIRA; RODRIGO PORTES URESHINO; CLAUDIA BINCOLETTO; GUSTAVO JOSÉ DA SILVA PEREIRA; SORAYA SOUBHI SMAILI. Efeitos do antagonista seletivo do REα e do REβ ICI 182,780 e do agonista de GPER G-1 no processo de autofagia em células de câncer de mama. Einstein (São Paulo), v. 18, 2020-04-22. (13/20073-2, 08/11515-3)

URESHINO, RODRIGO PORTES; HSU, YI-TE; DO CARMO, LUCIA GARCEZ; YOKOMIZO, CESAR HENRIQUE; NANTES, ISELI LOURENCO; SMAILI, SORAYA SOUBHI. Inhibition of cytoplasmic p53 differentially modulates Ca2+ signaling and cellular viability in young and aged striata. Experimental Gerontology, v. 58, p. 120-127, OCT 2014. (13/20073-2, 12/08273-3)

URESHINO, RODRIGO PORTES; ERUSTES, ADOLFO GARCIA; BASSANI, TAYSA BERVIAN; WACHILEWSKI, PATRICIA; GUARACHE, GABRIEL CICOLIN; NASCIMENTO, ANA CAROLINA; COSTA, ANGELICA JARDIM; SMAILI, SORAYA SOUBHI; DA SILVA PEREIRA, GUSTAVO JOSE. The Interplay between Ca2+ Signaling Pathways and Neurodegeneration. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 23, DEC 2019. (16/20796-2, 17/23616-8, 13/20073-2, 17/10863-7)

ANTUNES, FERNANDA; PEREIRA, GUSTAVO J. S.; SAITO, RENATA F.; BURI, V, MARCUS; GAGLIARDI, MARA; BINCOLETTO, CLAUDIA; CHAMMAS, ROGER; FIMIA, GIAN MARIA; PIACENTINI, MAURO; CORAZZARI, MARCO; et al. Effective Synergy of Sorafenib and Nutrient Shortage in Inducing Melanoma Cell Death through Energy Stress. CELLS, v. 9, n. 3, MAR 2020. (13/20073-2, 17/10863-7, 12/08273-3)

NASCIMENTO, ANA CAROLINA; ERUSTES, ADOLFO G.; RECKZIEGEL, PATRICIA; BINCOLETTO, CLAUDIA; URESHINO, RODRIGO P.; PEREIRA, GUSTAVO J. S.; SMAILI, SORAYA S.. alpha-Synuclein Overexpression Induces Lysosomal Dysfunction and Autophagy Impairment in Human Neuroblastoma SH-SY5Y. Neurochemical Research, v. 45, n. 11, p. 13-pg., 2020-09-11. (13/20073-2, 19/02821-8, 17/10863-7, 16/20796-2)

ANTUNES, FERNANDA; ERUSTES, ADOLFO GARCIA; COSTA, ANGELICA JARDIM; NASCIMENTO, ANA CAROLINA; BINCOLETTO, CLAUDIA; URESHINO, RODRIGO PORTES; SILVA PEREIRA, GUSTAVO JOSE; SMAILI, SORAYA SOUBHI. Autophagy and intermittent fasting: the connection for cancer therapy?. Clinics, v. 73, p. 7-pg., 2018-01-01. (08/11515-3, 13/20073-2)

NASCIMENTO, ANA CAROLINA; ERUSTES, ADOLFO G.; RECKZIEGEL, PATRICIA; BINCOLETTO, CLAUDIA; URESHINO, RODRIGO P.; PEREIRA, GUSTAVO J. S.; SMAILI, SORAYA S.. alpha-Synuclein Overexpression Induces Lysosomal Dysfunction and Autophagy Impairment in Human Neuroblastoma SH-SY5Y. Neurochemical Research, SEP 2020. (19/02821-8, 16/20796-2, 13/20073-2, 17/10863-7)

PEREIRA, GUSTAVO J. S.; ANTONIOLI, MANUELA; HIRATA, HANAKO; URESHINO, RODRIGO P.; NASCIMENTO, ALINE R.; BINCOLETTO, CLAUDIA; VESCOVO, TIZIANA; PIACENTINI, MAURO; FIMIA, GIAN MARIA; SMAILI, SORAYA S.. Glutamate induces autophagy via the two-pore channels in neural cells. ONCOTARGET, v. 8, n. 8, p. 12730-12740, 2017. (08/11515-3, 13/01769-6, 13/20073-2)

ANTUNES, F.; PEREIRA, G. J.; JASIULIONIS, M. G.; BINCOLETTO, C.; SMAILI, S. S.. Nutritional shortage augments cisplatin-effects on murine melanoma cells. Chemico-Biological Interactions, v. 281, p. 89-97, FEB 1 2018. (12/51215-4, 13/20073-2)

URESHINO, RODRIGO PORTES; COSTA, ANGELICA JARDIM; ERUSTES, ADOLFO GARCIA; DA SILVA PEREIRA, GUSTAVO JOSE; SINIGAGLIA-COIMBRA, RITA; SMAILI, SORAYA SOUBHI. Effects of Aging in the Striatum and Substantia Nigra of a Parkinson's Disease Animal Model. TOXICOLOGIC PATHOLOGY, v. 46, n. 3, p. 348-358, APR 2018. (13/20073-2, 12/08273-3)

COSTA, ANGELICA JARDIM; ERUSTES, ADOLFO GARCIA; SINIGAGLIA, RITA; NEVES GIRARDI, CARLOS EDUARDO; DA SILVA PEREIRA, GUSTAVO JOSE; URESHINO, RODRIGO PORTES; SMAILI, SORAYA SOUBHI. Lack of Autophagy Induction by Lithium Decreases Neuroprotective Effects in the Striatum of Aged Rats. PHARMACEUTICS, v. 13, n. 2, FEB 2021. (17/10863-7, 12/08273-3, 16/20796-2, 13/20073-2, 13/20976-2)

ERUSTES, ADOLFO GARCIA; D'ELETTO, MANUELA; GUARACHE, GABRIEL CICOLIN; URESHINO, RODRIGO PORTES; BINCOLETTO, CLAUDIA; DA SILVA PEREIRA, GUSTAVO JOSE; PIACENTINI, MAURO; SMAILI, SORAYA SOUBHI. Overexpression of alpha-synuclein inhibits mitochondrial Ca2+ trafficking between the endoplasmic reticulum and mitochondria through MAMs by altering the GRP75-IP3R interaction. Journal of Neuroscience Research, v. 99, n. 11, SEP 2021. (19/02821-8, 16/05580-3, 17/10863-7, 13/20073-2, 19/14722-4, 16/20796-2)

MULER, MARI LUMINOSA; ANTUNES, FERNANDA; GUARACHE, GABRIEL CICOLIN; OLIVEIRA, RAFAELA BRITO; URESHINO, RODRIGO PORTES; BINCOLETTO, CLAUDIA; DA SILVA PEREIRA, GUSTAVO JOSE; SMAILI, SORAYA SOUBHI. Effects of ICI 182,780, an ER alpha and ER beta antagonist, and G-1, a GPER agonist, on autophagy in breast cancer cells. Einstein (São Paulo), v. 18, 2020. (13/20073-2, 08/11515-3)