A systemic approach to study permissivity on the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV)

• Grant number: 14/17766-9
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2015
• End date: April 30, 2017
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Paolo Marinho de Andrade Zanotto
• Grantee: Paolo Marinho de Andrade Zanotto
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Baculoviridae are arthropod-specific viruses that commonly infect insect orders. During the viral life cycle, two distinct phenotypes are produced: the budded virus (BV) and, the occlusion-derived virus (ODV) for intra and across host spread, respectively. Since the 80's several countries have been using the Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) as a biological control agent against the velvet bean caterpillar (A. gemmatalis). In Brazil since the 1980's have been using AgMNPV in the world largest biological control program in soybean plantations. Our previous work revealed that the AgMNPV-2D genome carries at least 152 potential genes and that 149 viral genes are expressed during the infection. Moreover we also showed that a total of 44 proteins are associated to the ODV and 33 to the BV of the AgMNPV-2D. Furthermore, 11 cellular proteins were also identified, which are possibly assorted during viral morphogenesis and carried with the virion. However, when comparing the BV phenotype produced in semi permissive cells, we identified some differences. For example, the late and low level of expression of some genes, and the absence of proteins: AG52, IAP - 2, AG73, P33, 38K, P40, P48 and AG113 in its structure. Therefore, this project aims to understand the mechanisms that influence cell permissiveness to AgMNPV in the cell lineage UFL-AG-286 and SF-9. In addition it aims to understand the differences and dynamics of formation of new BV phenotype in permissive and semi-permissive cell lineage. For this, the specific goals are: (i) identify and quantify all viral and host mRNAs expressed in the early infection in different cell lines of Lepidoptera (ii) test the activity of the anti-apoptotic protein originating from the caterpillar Lonomia oblique to assess changes in permissivity (iii) map the ORFs in silico and compare the differences, (iv) quantify BV proteins based on mass spectrometry using label free methods (v) validate the data and propose pathways and signaling / interaction networks, (vi) to estimate genetic diversity measures (¸) from 17 South-American natural populations of AgMNPV, (vii) to infer evolutionary process based on neutrality tests on the 17 populations, (viii) to measure population differentiation and (iv) to correlate genetic population statistics to measures from genic networks. Permissivity is the main relationship between host-parasite relationships as specificity. Understanding how this process works can help searching for new baculovirus able to be used as biological control, as well as enabling possible future applications such as antigen presentation, alteration of cell tropism, gene delivery and expansion of host range. (AU)

Scientific publications

(23)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RUSSO, FABIELE BALDINO; BALEEIRO BELTRAO-BRAGA, PATRICIA CRISTINA. The impact of Zika virus in the brain. Biochemical and Biophysical Research Communications, v. 492, n. 4, SI, p. 603-607, OCT 28 2017. (14/17766-9, 11/18703-2)

DI PAOLA, NICHOLAS; CUNHA, MARIELTON DOS PASSOS; DURIGON, GIULIANA STRAVINSKAS; BEREZIN, EITAN NAAMAN; DURIGON, EDISON; LEAL DE OLIVEIRA, DANIELLE BRUNA; DE ANDRADE ZANOTTO, PAOLO MARINHO. Complete Genome Sequences of Two Human Parainfluenza Virus Type 3 Isolates Collected in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 5, n. 49, DEC 2017. (14/17766-9, 16/08204-2, 13/22136-1)

DI PAOLA, NICHOLAS; CUNHA, MARIELTON DOS PASSOS; DURIGON, GIULIANA STRAVINSKAS; BEREZIN, EITAN NAAMAN; DURIGON, EDISON; LEAL DE OLIVEIRA, DANIELLE BRUNA; DE ANDRADE ZANOTTO, PAOLO MARINHO. Complete Genome Sequences of Five Human Respiratory Syncytial Virus Isolates Collected in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 7, FEB 2018. (14/17766-9, 16/08204-2, 13/22136-1)

IOSHINO, RAFAELLA SAYURI; CARVALHO, DANILO OLIVEIRA; SANTOS MARQUES, ISABEL CRISTINA; FERNANDES, EDIANE SARAIVA; CAPURRO, MARGARETH LARA; COSTA-DA-SILVA, ANDRE LUIS. Oviplate: A Convenient and Space-Saving Method to Perform Individual Oviposition Assays in Aedes aegypti. INSECTS, v. 9, n. 3, SEP 2018. (13/19921-9, 14/17766-9)

FALL, GAMOU; DI PAOLA, NICHOLAS; FAYE, MARTIN; DIA, MOUSSA; DE MELO FREIRE, CAIO CESAR; LOUCOUBAR, CHEIKH; DE ANDRADE ZANOTTO, PAOLO MARINHO; FAYE, OUSMANE; SALL, AMADOU ALPHA. Biological and phylogenetic characteristics of West African lineages of West Nile. PLoS Neglected Tropical Diseases, v. 11, n. 11, NOV 2017. (14/17766-9, 13/22136-1)

MAZUR, FERNANDO G.; MORINISI, LEANDRO M.; MARTINS, JUNIOR OLIMPIO; GUERRA, PEDRO PONTES BUENO; FREIRE, CAIO C. M.. xploring Virome Diversity in Public Data in South America as an Approach for Detecting Viral Sources From Potentially Emerging Viruse. FRONTIERS IN GENETICS, v. 12, JAN 21 2022. (14/17766-9, 14/06090-4)

DI PAOLA, NICHOLAS; MESQUITA, FLAVIO S.; LEAL DE OLIVEIRA, DANIELLE BRUNA; VILLABONA-ARENAS, CHRISTIAN JULIAN; POUR, SHAHAB ZAKI; DE SOUSA-CAPRA, CARLA; LOPES, GABRIELA PESSANHA; FERRAZ SANTANA, RUBIA ANITA; REBELLO PINHO, JOAO RENATO; BALARINI, KARIME; et al. An Outbreak of Human Parvovirus B19 Hidden by Dengue Fever. Clinical Infectious Diseases, v. 68, n. 5, p. 810-817, MAR 1 2019. (14/17766-9, 15/25643-7, 13/22136-1)

LAROCCA, RAFAEL A.; ABBINK, PETER; PERON, JEAN PIERRE S.; ZANOTTO, PAOLO M. DE A.; IAMPIETRO, M. JUSTIN; BADAMCHI-ZADEH, ALEXANDER; BOYD, MICHAEL; NG'ANG'A, DAVID; KIRILOVA, MARINELA; NITYANANDAM, RAMYA; et al. Vaccine protection against Zika virus from Brazil. Nature, v. 536, n. 7617, p. 474+, AUG 25 2016. (14/17766-9, 11/18703-2)

ABBINK, PETER; LAROCCA, RAFAEL A.; DE LA BARRERA, RAFAEL A.; BRICAULT, CHRISTINE A.; MOSELEY, EDWARD T.; BOYD, MICHAEL; KIRILOVA, MARINELA; LI, ZHENFENG; NG'ANG'A, DAVID; NANAYAKKARA, OVINI; et al. Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys. Science, v. 353, n. 6304, p. 1129-1132, SEP 9 2016. (14/17766-9, 11/18703-2)

DI PAOLA, NICHOLAS; CUNHA, MARIELTON DOS PASSOS; DURIGON, GIULIANA STRAVINSKAS; BEREZIN, EITAN NAAMAN; DURIGON, EDISON; LEAL DE OLIVEIRA, DANIELLE BRUNA; DE ANDRADE ZANOTTO, PAOLO MARINHO. Complete Genome Sequences of Five Human Respiratory Syncytial Virus Isolates Collected in Brazil. GENOME ANNOUNCEMENTS, v. 6, n. 7, p. 2-pg., 2018-02-01. (14/17766-9, 16/08204-2, 13/22136-1)

RUSSO, FABIELE BALDINO; BALEEIRO BELTRAO-BRAGA, PATRICIA CRISTINA. The impact of Zika virus in the brain. Biochemical and Biophysical Research Communications, v. 492, n. 4, p. 5-pg., 2017-10-28. (14/17766-9, 11/18703-2)

DI PAOLA, NICHOLAS; CUNHA, MARIELTON DOS PASSOS; DURIGON, GIULIANA STRAVINSKAS; BEREZIN, EITAN NAAMAN; DURIGON, EDISON; LEAL OLIVEIRA, DANIELLE BRUNA; DE ANDRADE ZANOTTO, PAOLO MARINHO. Complete Genome Sequence of a Human Metapneumovirus Isolate Collected in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 6, FEB 2018. (14/17766-9, 16/08204-2, 13/22136-1)

CAIO CÉSAR DE MELO FREIRE; GIUSEPPE PALMISANO; CARLA T BRACONI; FERNANDA R CUGOLA; FABIELE B RUSSO; PATRICIA CB BELTRÃO-BRAGA; ATILA IAMARINO; DANIEL FERREIRA DE LIMA NETO; AMADOU ALPHA SALL; LIVIA ROSA-FERNANDES; et al. NS1 codon usage adaptation to humans in pandemic Zika virus. Memórias do Instituto Oswaldo Cruz, v. 113, n. 5, 2018-05-10. (14/03911-7, 16/03605-9, 14/17766-9, 12/04818-5, 14/06090-4)

BARBOSA, CARLA M.; DI PAOLA, NICHOLAS; CUNHA, MARIELTON P.; RODRIGUES-JESUS, MONICA J.; ARAUJO, DANIELLE B.; SILVEIRA, VANESSA B.; LEAL, FABYANO B.; MESQUITA, FLAVIO S.; BOTOSSO, VIVIANE F.; ZANOTTO, PAOLO M. A.; et al. Yellow Fever Virus RNA in Urine and Semen of Convalescent Patient, Brazil. Emerging Infectious Diseases, v. 24, n. 1, p. 176-178, JAN 2018. (14/17766-9, 14/21141-4, 16/08727-5, 13/22136-1, 16/08204-2)

COSTA-DA-SILVA, ANDRE LUIS; IOSHINO, RAFAELLA SAYURI; PETERSEN, VIVIAN; LIMA, ANTONIO FERNANDO; CUNHA, MARIELTON DOS PASSOS; WILEY, MICHAEL R.; LADNER, JASON T.; PRIETO, KARLA; PALACIOS, GUSTAVO; COSTA, DANUZA DUARTE; et al. First report of naturally infected Aedes aegypti with chikungunya virus genotype ECSA in the Americas. PLoS Neglected Tropical Diseases, v. 11, n. 6, JUN 2017. (13/19921-9, 16/08204-2, 14/17766-9, 14/27172-9)

CUGOLA, FERNANDA R.; FERNANDES, ISABELLA R.; RUSSO, FABIELE B.; FREITAS, BEATRIZ C.; DIAS, JOAO L. M.; GUIMARAES, KATIA P.; BENAZZATO, CECILIA; ALMEIDA, NATHALIA; PIGNATARI, GRACIELA C.; ROMERO, SARAH; et al. The Brazilian Zika virus strain causes birth defects in experimental models. Nature, v. 534, n. 7606, p. 267+, JUN 9 2016. (14/17766-9, 11/18703-2)

DOMBROWSKI, JAMILLE GREGORIO; DE SOUZA, RODRIGO MEDEIROS; LIMA, FLAVIA AFONSO; BANDEIRA, CARLA LETICIA; MURILLO, OSCAR; COSTA, DOUGLAS DE SOUSA; MACHADO PEIXOTO, ERIKA PAULA; CUNHA, MARIELTON DOS PASSOS; DE ANDRADE ZANOTTO, PAOLO MARINHO; BEVILACQUA, ESTELA; et al. Association of Malaria Infection During Pregnancy With Head Circumference of Newborns in the Brazilian Amazon. JAMA NETWORK OPEN, v. 2, n. 5, MAY 2019. (14/17766-9, 18/20468-0, 12/04755-3, 13/21728-2, 14/20451-0, 14/09964-5, 15/06106-0, 13/00981-1, 16/08204-2, 13/16417-8, 09/53889-0)

MESCI, PINAR; MACIA, ANGELA; LAROCK, CHRISTOPHER N.; TEJWANI, LEON; FERNANDES, ISABELLA R.; SUAREZ, NICOLE A.; ZANOTTO, PAOLO M. DE A.; BELTRAO-BRAGA, PATRICIA C. B.; NIZET, VICTOR; MUOTRI, ALYSSON R.. Modeling neuro-immune interactions during Zika virus infection. Human Molecular Genetics, v. 27, n. 1, p. 41-52, JAN 1 2018. (14/17766-9, 11/18703-2)

DI PAOLA, NICHOLAS; CUNHA, MARIELTON DOS PASSOS; DURIGON, GIULIANA STRAVINSKAS; BEREZIN, EITAN NAAMAN; DURIGON, EDISON; LEAL DE OLIVEIRA, DANIELLE BRUNA; DE ANDRADE ZANOTTO, PAOLO MARINHO. Complete Genome Sequences of Two Human Parainfluenza Virus Type 3 Isolates Collected in Brazil. GENOME ANNOUNCEMENTS, v. 5, n. 49, p. 2-pg., 2017-12-01. (14/17766-9, 16/08204-2, 13/22136-1)

DI PAOLA, NICHOLAS; CUNHA, MARIELTON DOS PASSOS; DURIGON, GIULIANA STRAVINSKAS; BEREZIN, EITAN NAAMAN; DURIGON, EDISON; LEAL OLIVEIRA, DANIELLE BRUNA; DE ANDRADE ZANOTTO, PAOLO MARINHO. Complete Genome Sequence of a Human Metapneumovirus Isolate Collected in Brazil. GENOME ANNOUNCEMENTS, v. 6, n. 6, p. 2-pg., 2018-02-01. (14/17766-9, 16/08204-2, 13/22136-1)

OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; MENDES, ERICA A.; LADNER, JASON T.; ANDREATA-SANTOS, ROBERT; ARAUJO, DANIELLE B.; BOTOSSO, VIVIANE F.; PAOLA, NICHOLAS D.; NETO, DANIEL F. L.; CUNHA, MARIELTON P.; et al. Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System. Viruses-Basel, v. 10, n. 11, NOV 2018. (13/07467-1, 14/17766-9, 14/27228-4, 14/16333-1, 16/08727-5, 15/25643-7, 14/03911-7, 16/08204-2, 16/20045-7, 13/26942-2, 16/03605-9)

CUNHA, MARIELTON DOS PASSOS; DOS SANTOS, CLIOMAR ALVES; DE LIMA NETO, DANIEL FERREIRA; SCHANOSKI, ALESSANDRA SOARES; POUR, SHAHAB ZAKI; PASSOS, SAULO DUARTE; FEITOSA DE SOUZAE, MERCIA SIMONE; COSTA, DANUZA DUARTE; DE ANDRADE ZANOTTO, PAOLO MARINHO. Outbreak of chikungunya virus in a vulnerable population of Sergipe, Brazil-A molecular and serological survey. Journal of Clinical Virology, v. 97, p. 44-49, DEC 2017. (16/08204-2, 14/17766-9, 16/03605-9)

COSTA-DA-SILVA, ANDRE LUIS; IOSHINO, RAFAELLA SAYURI; CORREA DE ARAUJO, HELENA ROCHA; KOJIN, BIANCA BURINI; DE ANDRADE ZANOTTO, PAOLO MARINHO; LEAL OLIVEIRA, DANIELLE BRUNA; MELO, STELLA REZENDE; DURIGON, EDISON LUIZ; CAPURRO, MARGARETH LARA. Laboratory strains of Aedes aegypti are competent to Brazilian Zika virus. PLoS One, v. 12, n. 2, FEB 10 2017. (13/19921-9, 14/17766-9, 13/16609-4)